| 球孢白僵菌Bbchitinase 1和Bbchitinase 2在侵染宿主过程中的不同作用 |
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| 引用本文： | 汪永松,耿涛,卢芙萍,武华周,娄德钊,涂娜娜,王树昌.球孢白僵菌Bbchitinase 1和Bbchitinase 2在侵染宿主过程中的不同作用[J].热带作物学报,2021,42(4):1092-1098. |
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| 作者姓名： | 汪永松 耿涛 卢芙萍 武华周 娄德钊 涂娜娜 王树昌 |
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| 作者单位： | 1.海南大学,海南海口 5702282.中国热带农业科学院环境与植物保护研究所,海南海口 571101 |
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| 基金项目： | 国家桑蚕产业技术体系海口综合试验站项目(CARS-18-SYZ17);中央级公益性科研院所基本科研业务费专项(1630042020007);中央级公益性科研院所基本科研业务费专项(1630042020013) |
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| 摘 要： | 球孢白僵菌（Beauveria bassiana）在宿主昆虫体壁穿透和体内定植过程中分泌了Bbchitinase 1和Bbchitinase 2两种胞外几丁质酶,但二者的作用分工尚不明确。本文通过菌株胞外总几丁质酶活性与侵染毒力的相关性分析,Bbchitinase 1和Bbchitinase 2基因表达水平与侵染毒力的相关性分析,Bbchitinase 1和Bbchitinase 2的生物信息学分析以及在侵染过程中的表达模式分析,以期明晰Bbchitinase 1和Bbchitinase 2在菌株侵染宿主过程中的作用分工。结果表明,菌株胞外几丁质酶活性与侵染毒力呈正相关,但Bbchitinase 1和Bbchitinase 2表达水平不同。菌株间Bbchitinase 1表达水平与菌株侵染毒力（MMT和LT50）呈显著正相关,而Bbchitinase 2表达水平却与侵染毒力不相关。生物信息学分析结果显示,Bbchitinase 1和Bbchitinase 2具有显著的结构和功能差异,可能结合不同的配体蛋白,参与不同的代谢过程。表达模式分析结果表明,Bbchitinase 1和Bbchitinase 2基因在侵染宿主过程中均上调表达;但Bbchitinase 1仅在侵染家蚕的体壁穿透阶段显著上调表达,而Bbchitinase 2在芽生孢子生成阶段和菌丝大量增殖阶段显著上调表达。以上研究结果说明,Bbchitinase 1可能参与菌株侵染早期的侵入钉形成和体壁穿透过程,与侵染毒力呈正相关;而Bbchitinase 2可能参与侵染过程中的芽生孢子发育和菌丝大量增殖代谢调控。本研究明晰了球孢白僵菌不同几丁质酶在侵染过程中的作用分工,建立了以Bbchitinase 1基因表达水平判定菌株毒力的数学模型,为高毒、高稳定性球孢白僵菌生防菌株的筛选提供重要科学依据,对提升绿色生态防治应用效果具有重要意义。
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| 关 键 词： | 球孢白僵菌 毒力因子 几丁质酶 家蚕 |
| 收稿时间： | 2020-04-01 |
Different Roles of Beauveria bassiana Bbchitinase 1 and Bbchitinase 2 in Host Infection |
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| WANG Yongsong,GENG Tao,LU Fuping,WU Huazhou,LOU Dezhao,TU Nana,WANG Shuchang.Different Roles of Beauveria bassiana Bbchitinase 1 and Bbchitinase 2 in Host Infection[J].Chinese Journal of Tropical Crops,2021,42(4):1092-1098. |
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| Authors: | WANG Yongsong GENG Tao LU Fuping WU Huazhou LOU Dezhao TU Nana WANG Shuchang |
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| Institution: | 1. Hainan University, Haikou, Hainan 570228, China2. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China |
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| Abstract: | Two extracellular chitinases were secreted by Beauveria bassiana during the infection process, named as Bbchitinase 1 and Bbchitinase 2, but the roles were not clear yet. The correlation analysis between chitinase activity and virulence, correlation analysis between gene expression level and virulence, bioinformatics analysis and expression patterns assay were performed in this paper in order to clarify different roles of Bbchitinase 1 and Bbchitinase 2. Statistical analysis and inhibition test showed that extracellular chitinase activitiy was positively correlated with the virulence, while the expression levels of Bbchitinase 1 and Bbchitinase 2 were different in strains. The expression level of Bbchitinase 1 was positively correlated with the virulence of the strains (MMT and LT50); meanwhile, there was no significant relationship between Bbchitinase 1 and virulence of the strains. In addition, the bioinformatics analysis showed that there were significant differences in structure and function of Bbchitinase 1 and Bbchitinase 2, which might combine with different ligands and participate in different metabolic processes. Furthermore, the spatiotemporal expression pattern analysis showed that Bbchitinase 1 was significantly up-regulated only in the formation of penetration peg and penetration of perisoma stage of infection, meanwhile Bbchitinase 2 was slightly up-regulated in the formation of blastospore stage and significantly up-regulated in the mycelial vegetative growth stage. The results suggested that Bbchitinase 1 might be involved into body wall penetration at early infection stage and had positive correlation with virulence, while Bbchitinase 2 into the proliferation and metabolism of mycelium at the later infection stage. The results of this study clarified the role of different chitinases in the infection process of B. bassiana, and established a mathematical model to determine the virulence of B. bassiana based on the expression level of Bbchitinase 1 gene, which provides the important scientific basis for screening of high toxicity and high stability biocontrol strains and great significance for improving the application effect of green ecological control. |
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| Keywords: | Beauveria bassiana virulence factor chitinase Bombyx mori |
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