| 辣椒新叶斑病病原鉴定及其生物学特性 |
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| 引用本文： | 裴月令,孙燕芳,冯推紫,陈园,龙海波.辣椒新叶斑病病原鉴定及其生物学特性[J].热带作物学报,2021,42(9):2659-2665. |
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| 作者姓名： | 裴月令 孙燕芳 冯推紫 陈园 龙海波 |
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| 作者单位： | 中国热带农业科学院环境与植物保护研究所/农业农村部热带作物有害生物综合治理重点实验室/海南省热带农业有害生物监测与控制重点实验室,海南海口 571101 |
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| 基金项目： | 国家重点研发计划项目(2018YFD0201209);农业农村部部门预算项目“粮经轮作模式关键技术优化提升与集成示范”;中央级公益性科研院所基本科研业务费专项(1630042017003) |
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| 摘 要： | 2015—2019年,笔者在对海南地区的辣椒病害进行调查时,发现一种和常见病害症状不同的新叶斑病。田间采集病样后,经病原菌分离、显微形态观察和分子生物学鉴定,证明该病由新月弯孢(Curvularia lunata (Wakker) Boedijin]侵染引起,这是该种病原菌在中国危害辣椒的首次报道。生物学特性研究结果表明,玉米粉、30 ℃、pH 7.0、连续光照或光暗交替、麦芽糖和酵母提取物为病原菌最适培养条件,分生孢子最适萌发温度和致死温度分别为22~33 ℃和60 ℃（10 min）。12种杀菌剂的室内药剂筛选结果表明,45%咪鲜胺EW抑菌效果最好,浓度为1.0 mg/L时抑菌率达97.8%。16%乙霉威·10%嘧霉胺WP、25%三唑酮WP、65%代森锌WP和80%代森锰锌WP也有较好的抑菌效果,而70%甲基硫菌灵WP、80%多菌灵WP几乎无抑菌作用。
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| 关 键 词： | 辣椒 新月弯孢 生物学特性 药剂筛选 |
| 收稿时间： | 2020-09-01 |
Pathogen Identification for the New Leaf Spot Disease of Capsicum annuum L. and Its Biological Characteristics |
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| PEI Yueling,SUN Yanfang,FENG Tuizi,CHEN Yuan,LONG Haibo.Pathogen Identification for the New Leaf Spot Disease of Capsicum annuum L. and Its Biological Characteristics[J].Chinese Journal of Tropical Crops,2021,42(9):2659-2665. |
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| Authors: | PEI Yueling SUN Yanfang FENG Tuizi CHEN Yuan LONG Haibo |
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| Institution: | Environment and Plant Protection Institute, Chinese Academy of Tropical Agriculture Sciences / Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs / Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China |
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| Abstract: | A new leaf spot disease was firstly observed during a survey from 2015—2019 in Capsicμm annuμm field of Hainan Province, which was different with other common leaf diseases. The sample was collected and the pathogen was isolated. The results of microscopic examination and molecular analysis confirmed that the disease was caused by Curvularia lunata (Wakker) Boedijin, which was the first report of the Capsicum disease caused by C. lunata in China. The optimum condition for mycelial growth was 30 ℃, pH 7, light or alternative light, D-maltose and yeast extract, while the optimum temperature for conidia germination was 22-33 ℃ and the lethal temperature for conidia was 60 ℃ for 10 min. 12 fungicides were screened in laboratory, 45% Prochloraz EW was found to have the best inhibition activity against the pathogen, which had 97.8% inhibition when the concentration was 1.0 mg/L, 16% diethofencarb 10% pyrimethanil WP, 25% triadimefon WP, 65% Tiezene WP and 80% Mancozeb WP were also effective, while 70% Thiophanate-methyl and 80% carbendazim were almost futile. |
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| Keywords: | Capsicum annuum L Curvularia lunata biological characteristics fungicide screening |
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