| 橡胶树‘热研7-33-97’死皮相关研究的qRT-PCR内参基因筛选 |
| |
| 引用本文： | 卢亚莉,张世鑫,杨署光,田维敏,史敏晶.橡胶树‘热研7-33-97’死皮相关研究的qRT-PCR内参基因筛选[J].热带作物学报,2021,42(5):1282-1289. |
| |
| 作者姓名： | 卢亚莉 张世鑫 杨署光 田维敏 史敏晶 |
| |
| 作者单位： | 中国热带农业科学院橡胶研究所/农业农村部橡胶树生物学与遗传资源利用重点实验室/省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室,海南海口 571101 |
| |
| 基金项目： | 国家重点研发计划项目(2018YFD1000502);国家自然科学基金项目(31870590);现代农业产业技术体系建设专项(CARS-33-YZ1) |
| |
| 摘 要： | 在橡胶树死皮以及死皮康复相关研究中,为获得可靠的基因表达结果,筛选合适的内参基因显得尤为重要。本研究以‘热研7-33-97’健康树、三级死皮树和死皮康复树为试验材料,收集其中的胶乳,采用qRT-PCR技术,结合GeNorm、NormFinder和BestKeeper三种内参筛选软件,综合分析了20个候选内参基因在不同胶乳样品中的表达情况。结果表明：eif2、ACT7a、UBC2b在健康树中较稳定;UBC3、eifAb、eifAa、ADF4、UBC4、UBC2b、eif2、RH2b、ACT7b在三级死皮树中较稳定;UBC2b、eif2和ROC3在三级死皮恢复树中较稳定。选择胶乳代谢相关基因HbDXS1、HbHMGS2和HbNIN3对候选的内参基因进行验证,筛选出本研究中适合的一组稳定可靠的内参基因是eif2和UBC2b,其中ejf2是最佳内参基因,18S不适合作为本研究的内参基因。
|
| 关 键 词： | 橡胶树 死皮相关 内参基因 qRT-PCR |
| 收稿时间： | 2020-05-19 |
Selection of reference genes for qRT-PCR in TPD-related Study of ‘Reyan 7-33-97’ in Rubber Tree (Hevea brasiliensis Muell. Arg.) |
| |
| LU Yali,ZHANG Shixin,YANG Shuguang,TIAN Weimin,SHI Minjing.Selection of reference genes for qRT-PCR in TPD-related Study of ‘Reyan 7-33-97’ in Rubber Tree (Hevea brasiliensis Muell. Arg.)[J].Chinese Journal of Tropical Crops,2021,42(5):1282-1289. |
| |
| Authors: | LU Yali ZHANG Shixin YANG Shuguang TIAN Weimin SHI Minjing |
| |
| Institution: | Rubber Research Institute, CATAS / Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs / State Key Laboratory Incubation Base for Cultivation & Physiology of Tropical Crops, Haikou, Hainan, 571101, China |
| |
| Abstract: | In order to obtain reliable gene expression results for TPD-related study of rubber tree, the selection of suitable reference genes is very important. In the present study, using the latex from the healthy rubber tree, the third grade tapping panel dryness (TPD) tree and the TPD-recovering tree of ‘Reyan7-33-97’ as the experimental materials, the expression stability of 20 candidate reference genes were evaluated by using quantitative real-time PCR (qTR-PCR) and softwares of geNorm, NormFinder and BestKeeper. The results showed that, reference genes eif2、ACT7a and UBC2b were the top three stable genes in the healthy tree; UBC3、eifAb、eifAa、ADF4、UBC4、UBC2b、eif2、RH2b and ACT7b were all stable genes in the third grade TPD tree; UBC2b、eif2 and ROC3 were stable genes for the TPD-recovering rubber tree. Based on the different stable reference genes, relative expressions of HbDXS1, HbHMGS2 and HbNIN3 genes were analyzed respectively. The results showed that eif2 and UBC2b were a couple of stable reference genes for TPD-related study; the eif2 was the most stable internal reference gene; 18S gene was not suitable as reference gene for qTR-PCR in this study. |
| |
| Keywords: | Hevea brasiliensis Muell Arg TPD-related reference genes qTR-PCR |
|
| 点击此处可从《热带作物学报》浏览原始摘要信息 |
| 点击此处可从《热带作物学报》下载免费的PDF全文 |
|
