| 利用QTL-seq定位萝卜肉质根根形指数QTL |
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| 引用本文： | 胡天华,魏庆镇,汪精磊,王五宏,胡海娇,严亚琴,包崇来.利用QTL-seq定位萝卜肉质根根形指数QTL[J].浙江农业学报,2021,33(12):2313. |
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| 作者姓名： | 胡天华 魏庆镇 汪精磊 王五宏 胡海娇 严亚琴 包崇来 |
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| 作者单位： | 浙江省农业科学院 蔬菜研究所,浙江 杭州 310021 |
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| 基金项目： | 国家现代农业产业技术体系建设专项资金—萝卜(CARS-23-A14);浙江省基础公益研究计划(LGN19C060002) |
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| 摘 要： | 为检测萝卜肉质根根形指数相关的数量性状遗传位点(QTL),挖掘调控萝卜肉质根根形的关键基因,以肉质根根形指数差异显著的小型扁圆萝卜LLYH和大型长白萝卜CLA为亲本,杂交构建了F2分离群体,利用QTL-seq方法对肉质根根形指数性状进行了QTL定位分析。一共检测到4个QTL位点,其中两个主效QTL位点rs2.1和rs2.2,分别位于萝卜R2号染色体21.66~26.03 Mb和30.79~36.56 Mb区域,两个微效QTL位点rs4.1和rs6.1,分别位于萝卜R4号染色体39.04~40.43 Mb和R6号染色体11.53~13.40 Mb。研究结果为开发与萝卜肉质根根形指数连锁的分子标记奠定了基础,同时为挖掘肉质根根形指数调控基因提供了重要参考。
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| 关 键 词： | 萝卜 根形指数 QTL-seq 数量性状遗传位点 |
| 收稿时间： | 2020-06-09 |
Identification of quantitative trait loci controlling radish root index by QTL-seq |
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| HU Tianhua,WEI Qingzhen,WANG Jinglei,WANG Wuhong,HU Haijiao,YAN Yaqin,BAO Chonglai.Identification of quantitative trait loci controlling radish root index by QTL-seq[J].Acta Agriculturae Zhejiangensis,2021,33(12):2313. |
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| Authors: | HU Tianhua WEI Qingzhen WANG Jinglei WANG Wuhong HU Haijiao YAN Yaqin BAO Chonglai |
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| Institution: | Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China |
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| Abstract: | In order to detect QTL loci and find the key genes related to radish root shape index, an F2 population was constructed by crossing the small-sized oblate spheroidal radish LLYH and big-sized long white radish CLA which had significant differences in root shape index. QTL mapping for root shape index traits was conducted using QTL-Seq method. Four QTL loci were detected, of which two major QTL loci rs2.1 and rs2.2 were located in the 21.66-26.03 Mb and 30.79-36.56 Mb regions on chromosome R2, respectively; two minor QTL loci rs4.1 and rs6.1 were located on chromosome R4 39.04-40.43 Mb and R6 11.53-13.40 Mb.These results laid a foundation for the development of molecular markers linked to root shape index and provided an important reference for fine mapping the regulatory genes of the root shape index. |
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| Keywords: | radish root index QTL-seq QTL |
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