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Expressed sequence tags from the phytopathogenic fungus Botrytis cinerea |
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| Authors: | Muriel Viaud Fabrice Legeai Jean-Marc Pradier Yves Brygoo Frédérique Bitton Jean Weissenbach Adeline Brunet-Simon Aymeric Duclert Sabine Fillinger Dominique Fortini Anastasia Gioti Corinne Giraud Sébastien Halary Isabelle Lebrun Pascal Le Pêcheur Delphine Samson Caroline Levis |
| Institution: | (1) Unité PMDV, INRA, Route de Saint-Cyr, Versailles, France, 78086;(2) URGI, INRA, Infobiogen, 523 Place des Terasses, Evry, France, 91000;(3) Génoscope, UMR CNRS 8030, 2 rue Gaston Crémieux, Evry, France, 91057, CP 5706;(4) UPMC, INRA, Route de Saint-Cyr, Versailles, France, 78086;(5) Present address: URGV, INRA, 2 rue Gaston Crémieux, Evry, France, 91000 |
| Abstract: | A large set of genes was identified in the phytopathogenic fungus Botrytis cinerea by using an expressed sequence tag approach. The fungus was grown in axenic culture and a cDNA library was produced. From this library, 6559 ESTs were obtained. The combined sequences represent 3026 unisequences that corresponds to approximately one-quarter of the estimated total number of genes in B. cinerea. Approximately 18% of the ESTs showed significant similarities with genes coding for proteins with known functions,~56% were similar to genes coding for proteins with unknown functions and ~26% were orphans. A substantial B. cinerea gene inventory including putative virulence factors was therefore obtained and is now available at the Génoplante-Info Database interface (http://urgi.infobiogen.fr///Projects/GPiDB/Interface/). |
| Keywords: | cDNA EST grey mould |
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