| 绿竹花发育相关基因BoAP3的克隆与分析 |
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| 引用本文： | 朱龙飞,徐英武,林新春.绿竹花发育相关基因BoAP3的克隆与分析[J].浙江林学院学报,2013(6):839-843. |
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| 作者姓名： | 朱龙飞 徐英武 林新春 |
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| 作者单位： | 浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江临安311300 |
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| 基金项目： | 国家自然科学基金资助项目（31000295）;国家重点基础研究发展计划（“973”计划）项目（2012CB723008） |
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| 摘 要： | MADS—box基因家族在决定花分生组织特性和花器官发育过程中起着重要的作用。以绿竹Bambusaoldhamii开花试管苗花芽为植物材料，采用cDNA末端快速扩增技术（rapid amplification of cDNAends，RACE）技术，获得了1条MADS—box基因家族的基因，命名为BoAP3。序列分析结果表明：BoAP3开放阅读框（open reading frame，ORF）长度为654bp，编码218个氨基酸，具有典型的植物MADS—box蛋白结构，其编码肽链包含了MADS区、K区、I区和C区。B胡丹与小麦Triticum aestivum，水稻Oryzasatva等AP3-like同源基因所编码的氨基酸同源性达到80％以上。定量聚合酶链式反应（PCR）结果表明：BoAP3基因在开花试管苗的花芽中表达量是不开花试管苗营养芽表达量的8．1倍，表明该基因可能参与了花器官的发育。
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| 关 键 词： | 林木遗传学 绿竹 BoAP3 MADS—box |
Cloning and analysis of the flowering-related gene,BoA P3, in Bambusa oldhamii |
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| ZHU Longfei,XU Yingwu,LIN Xinchun.Cloning and analysis of the flowering-related gene,BoA P3, in Bambusa oldhamii[J].Journal of Zhejiang Forestry College,2013(6):839-843. |
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| Authors: | ZHU Longfei XU Yingwu LIN Xinchun |
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| Institution: | (The Nurturing Station for the State Key Laboratory of Subtropical Silvicuhure, Zhejiang A ＆ F University, Lin＇an 311300, Zhejiang, China) |
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| Abstract: | MADS-box family genes play important roles in the determination of floral meristem identity and the development of floral organ identity. Here we reported a full length MADS-box gene obtained from the flower bud of Bambusa oldhamii. It was named as BoAP3 based on sequence similarity. DNA sequence analysis showed that the BoAP3 was 654 bp length and contained an open reading flarhe （ORF） encoding a prrtein of 218 mino acids that shared over 80% homology with Oryza sat＆a and Triticttm aestivum of APETALA3 homo- logues. The protein showed a typical MADS-box gene structure containing MADS domain, K domain, I region and C terminus. Quantitative real-time PCR anai.~sis revealed that the expression level of BoAP3 in flower buds was 8.1 times higher than in vegetative shoots, suggested that BoA t）3 may be involved in floral organ development. |
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| Keywords: | forest tree breeding Bambusa oldhamii BoA 193 MADS-box |
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