| IGF2基因在通城猪和长白猪胚胎骨骼肌中的表达研究 |
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| 引用本文： | 唐中林,李勇,邓宏,杨述林,牟玉连,储明星,马月辉,李奎.IGF2基因在通城猪和长白猪胚胎骨骼肌中的表达研究[J].农业生物技术学报,2008,16(2). |
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| 作者姓名： | 唐中林 李勇 邓宏 杨述林 牟玉连 储明星 马月辉 李奎 |
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| 作者单位： | 中国农业科学院北京畜牧兽医研究所 |
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| 摘 要： | IGF2基因对骨骼肌的生长发育起着非常重要的调控作用。为了研究IGF2对猪胚胎骨骼肌生长发育的调控，本文建立猪IGF2基因的SYBR-Green荧光定量PCR方法，并利用此方法分析该基因在通城猪（脂肪型）和长白猪（瘦肉型）妊娠33天、65天和90天胚胎骨骼肌中的表达规律。结果表明，建立的SYBR-Green荧光定量方法可以有效地用于IGF2的表达分析。IGF2基因在两品种中都以妊娠65天时表达水平最高，呈波浪式表达模式。有趣的是，在所研究的三个胚胎时期，IGF2的表达在通城猪中均高于长白猪，其原因值得深入研究。
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| 关 键 词： | 猪 胚胎骨骼肌 荧光定量PCR 胰岛素生长因子II 发育性变化 |
| 收稿时间： | 2007-8-16 |
| 修稿时间： | 2007-10-23 |
Study on the expression analysis of IGF2 gene in fetus skeletal muscle from Tongcheng and Landrace pigs |
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| Abstract: | IGF2 gene plays an important role in the development of skeletal muscle. In order to investigate the effect of IGF2 on the regulation of development of prenatal skeletal muscle in pigs, the SYBR-Green real-time PCR method was established and used for expression analysis of IGF2 in the prenatal skeletal muscles from Tongcheng and Landrace pigs at 33, 65 and 90 days post coitus (dpc). The results indicated that the real-time PCR method established in this study was effective for the expression analysis of IGF2 gene. IGF2 had the highest expression at mRNA level in skeletal muscle at 65 dpc among selected stages and exhibited the wave expression pattern in two breeds. Interestingly, the IGF2 expression was higher in Tongcheng than that in Landrace pigs at all three prenatal stages studied, but the reason is still unknown. |
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