| 夏枯草RuBPCase大亚基基因的克隆与表达 |
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| 引用本文： | 许锋,蒋丽阳,曹腾,宁迎晶,程水源.夏枯草RuBPCase大亚基基因的克隆与表达[J].贵州农业科学,2012,40(4):6-9. |
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| 作者姓名： | 许锋 蒋丽阳 曹腾 宁迎晶 程水源 |
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| 作者单位： | 1. 长江大学园艺园林学院,湖北荆州,434025
2. 黄冈师范学院经济林木种质改良与资源综合利用湖北省重点实验室,湖北黄冈,438000 |
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| 基金项目： | 国家大学生创新性实验计划项目 |
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| 摘 要： | 为从夏枯草中克隆参与光合作用和光呼吸代谢的关键酶RuBPcase大亚基基因rbcL,为今后研究夏枯草RuBPcase的结构和功能以及从分子水平探讨夏枯草的光合作用机理提供必要的基础数据,以夏枯草基因组DNA为模板,采用PCR法对夏枯草RuBPCase大亚基基因(PvrbcL)的克隆与表达进行研究。结果表明:PCR法扩增出夏枯草RuBPcase大亚基基因片段PvrbcL,基因长837bp,编码278个氨基酸,GenBank登录号为JN 692563;经BLAST序列比较,夏枯草PvrbcL基因核苷酸序列与大花夏枯草、鼠尾草、烟草、大豆、水稻、菜豆、葡萄、油菜的相似性为86%～100%,氨基酸同源性为92%～100%;Northern杂交分析表明,PvrbcL基因在夏枯草叶和茎中表达,且在叶中的表达量最大;发育表达模式结果显示,Pvr-bcL基因的表达量随着叶片的生长而增加。PvrbcL基因的克隆和表达模式分析为进一步研究夏枯草RuB-Pcase的结构和功能奠定了基础。
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| 关 键 词： | 夏枯草 RuBPcase的大亚基基因(rbcL) 克隆 表达 |
Molecular Cloning and Expression Analysis of A Large Subunit Gene of RuBPCase ( PvrbcL ) from Prunella vulgaris |
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| XU Feng
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JIANG Li-yang
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CAO Teng
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NING Ying-jing
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CHENG Shui-yuan.Molecular Cloning and Expression Analysis of A Large Subunit Gene of RuBPCase ( PvrbcL ) from Prunella vulgaris[J].Guizhou Agricultural Sciences,2012,40(4):6-9. |
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| Authors: | XU Feng JIANG Li-yang CAO Teng NING Ying-jing CHENG Shui-yuan |
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| Institution: | 1.College of Horticulture and Gardening,Yangtze University,Jingzhou,Hubei 434025;2.Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization,Huanggang Normal University,Huanggang,Hubei 438000,China) |
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| Abstract: | In order to clone the large subunit gene(rbcL) of Rubisco-1,5-bisphosphate carboxylase/oxylase(RuBPCase),one of the important enzyme involved in photosynthesis and photorespiration,from P.vulgaris,and so as to provide basic data for studying on the structure and function of RuBPCase and discussing the photosynthesis mechanism on the molecular level of P.vulgaris,a pair of specific primers was designed.An rbcL gene fragment,named PvrbcL,was cloned from P.vulgaris using genomic DNA as template.The results showed that the length of PvrbcL sequence was 837 bp,encoding a 278-amino-acid protein.The GenBank accession No.of PvrbcL gene was JN692563.The BLAST results showed that the homology of the PvrbcL nucleotide sequence with rbcL genes from Prunella grandiflora,Salvia pachyphylla,Nicotiana tabacum,Glycine max,Oryza sativa,Phaseolus vulgaris,Vitis vinifer,and Brassica napus was 86%~100%,and the homology of amino acid sequences was 92%~100%.Northern blot analysis revealed that PvrbcL gene expressed in leaves and stems,and the highest level was detected in leaves.Developmental expression patter indicated that the expression level of PvrbcL gene was increased along with the growth of the leaves.The isolation and expression of PvrbcL gene could provide the basis for further studying the structure and function of RuBPCase in P.vulgaris. |
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| Keywords: | Prunella vulgaris large subunit gene(rbcL) of Rubisco-1 5-bisphosphate carboxylase/oxylase cloning expression |
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