| 陕西卫矛组织培养再生体系的建立 |
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| 引用本文： | 袁云香,勾雪娇,冯辽辽,王志平,林娜,戚珍珠,赵小静.陕西卫矛组织培养再生体系的建立[J].贵州农业科学,2012,40(4):17-19. |
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| 作者姓名： | 袁云香 勾雪娇 冯辽辽 王志平 林娜 戚珍珠 赵小静 |
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| 作者单位： | 渭南师范学院化学与生命科学学院,陕西渭南,714000 |
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| 基金项目： | 国家自然科学基金，陕西省教育厅2009年度科学研究计划项目，渭南师范学院大学生创新计划项目 |
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| 摘 要： | 为建立陕西卫矛高效再生体系,以幼茎为外植体诱导愈伤组织,在成功继代愈伤组织的基础上,进行愈伤组织再分化培养研究。采用正交设计方法,考察了6-BA、NAA及Ca2+3个因素对陕西卫矛愈伤组织再分化率的影响。结果表明:再分化培养基中添加3mg/L 6-BA+0.3mg/L NAA+5mmol/L Ca2+,能明显提高陕西卫矛愈伤组织的再分化率。
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| 关 键 词： | 陕西卫矛 组织培养 愈伤组织 再分化率 再生体系 |
Establishment of Tissue Culture Regeneration System of Euonymus schensianus |
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| YUAN Yun-xiang
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GOU Xue-jiao
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FENG Liao-liao
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WANG Zhi-ping
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LIN Na
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QI Zhen-zhu
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ZHAO Xiao-jing.Establishment of Tissue Culture Regeneration System of Euonymus schensianus[J].Guizhou Agricultural Sciences,2012,40(4):17-19. |
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| Authors: | YUAN Yun-xiang GOU Xue-jiao FENG Liao-liao WANG Zhi-ping LIN Na QI Zhen-zhu ZHAO Xiao-jing |
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| Institution: | (College of Chemistry and Life Science,Weinan Teachers University,Weinan,Shaanxi 714000,China) |
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| Abstract: | In order to establish a high efficient regeneration system,the stems of E.schensianus were taken as explants and induced callus formation after having plenty proliferated calli successfully,and then the calli were placed on different combinations regeneration medium.An orthogonal test was carried out to observe the influences of the 6-BA,NAA and Ca2+ concentration on regeneration rate.The results showed that the suitable regeneration medium was MS containing 3.0 mg/L 6-BA,0.3 mg/L NAA and 5.0 mmol/L Ca2+,it could obviously improve the frequency of regenerated shoots. |
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| Keywords: | Euonymus schensianus tissue culture callus regeneration system |
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