| 微孔草籽粕氨基酸含量的测定与评价 |
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| 引用本文： | 周同永,邓黎,皮立,赵晓辉,韩涛,韩发.微孔草籽粕氨基酸含量的测定与评价[J].贵州农业科学,2012,40(4):51-55. |
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| 作者姓名： | 周同永 邓黎 皮立 赵晓辉 韩涛 韩发 |
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| 作者单位： | 1. 中国科学院西北高原生物研究所,青海西宁810008;中国科学院研究生院,北京100049;中国科学院高原生物适应与进化重点实验室,青海西宁810008
2. 中国科学院西北高原生物研究所,青海西宁810008;中国科学院高原生物适应与进化重点实验室,青海西宁810008 |
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| 基金项目： | 国家基金，科技部人员服务企业行动项目，中科院科技支青项目 |
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| 摘 要： | 为综合开发利用微孔草资源,以2,4-二硝基氟苯(DNFB)为衍生试剂,采用柱前衍生反相高效液相色谱法对微孔草籽粕的17种氨基酸含量进行了测定。结果表明:微孔草籽粕中各种氨基酸含量丰富,总含量达99.392mg/g,7种必需氨基酸含量为40.086mg/g,亮氨酸含量高达18.995mg/g;氨基酸配比合理,E/T为40.33%,E/N为0.676%,非常接近FAO/WHO提出的理想模式。超临界CO2萃取微孔草籽粕在医学和营养学上均具有较高的研究与开发价值。
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| 关 键 词： | 微孔草籽粕 氨基酸 反相高效液相色谱法 营养评价 |
Determination and Evaluation of Amino Acids Composition in Residues of Microula sikkimensis Seeds |
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| ZHOU Tong-yong
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DENG Li
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PI Li
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ZHAO Xiao-hui
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HAN Tao
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HAN Fa.Determination and Evaluation of Amino Acids Composition in Residues of Microula sikkimensis Seeds[J].Guizhou Agricultural Sciences,2012,40(4):51-55. |
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| Authors: | ZHOU Tong-yong DENG Li PI Li ZHAO Xiao-hui HAN Tao HAN Fa |
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| Institution: | 1,3 (1.Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,Qinghai 810008;2.Graduate University of Chinese Academy of Sciences,Beijing 100049;3.Key Laboratory of Adaptation and Evolution of Plateau Biota,China Academy of Sciences,Xining,Qinghai 810008,China) |
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| Abstract: | In order to make full use of M.sikkimensis,the highly sensitive fluorescent reagent and 2,4-dinitrofluorobenzene were applied to determine and evaluate the amino acid composition of M.sikkimensis seed residues(the seeds were extracted for seed oil by supercritical CO2 before) by pre-column derivatization method.The results showed that the total amino acid content reached 99.392 mg/g,moreover,the content of seven essential amino acids was 40.086 mg/g.Among them,Leu was the major amino acid,up to 18.995 mg/g.According to the guidance of FAO/WHO,the amino acid composition of M.sikkimensis was very valuable and nutritious(E/T: 40.331 %;E/N: 0.676).The samples was of high research potential at both medically and nutritionally. |
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| Keywords: | Microula sikkimensis amino acids RP-HPLC nutrition evaluation |
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