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| 烟草青枯菌土壤拮抗真菌的筛选及鉴定 | |
| 引用本文： | 陆铮铮,杨先权,彭杰,蒋选利,丁海霞,彭丽娟.烟草青枯菌土壤拮抗真菌的筛选及鉴定[J].贵州农业科学,2012,40(1):86-89,175. |
| 作者姓名： | 陆铮铮 杨先权 彭杰 蒋选利 丁海霞 彭丽娟 |
| 作者单位： | 1. 贵州大学贵州山地农业病虫害重点实验室,贵州贵阳550025;贵州大学农学院,贵州贵阳550025 2. 麻江县烟草局,贵州麻江,557600 3. 天柱县烟草局,贵州天柱,556600 4. 中国农业大学农学与生物技术学院,北京,100193 |
| 基金项目： | 中国烟草总公司贵州省公司科技项目 |
| 摘 要： | 为有效地利用生物防治技术防治烟草青枯病,提升烟草及烟草制品的产量和质量,解决使用化学防治造成的有害物质残留超标问题,通过采用平板对峙培养法、分子生物学与形态学相结合的生物防菌鉴定法等,对从健康的烟草根际土壤中分离获得的烟草青枯菌土壤拮抗真菌进行筛选和鉴定研究。结果表明:从烟草根际土壤中分离获得的56株真菌中有3株具有拮抗作用,经鉴定分别为淡色生赤壳菌(Bionec-tria ochroleuca)、棘孢木霉(Trichoderma asperellum)和嗜松青霉(Penicillium pinophilum),其拮抗效果从高到低依次为淡色生赤壳菌(平均抑菌圈直径达31.23mm)>棘孢木霉(11.28mm)>嗜松青(10.54mm)。 |
| 关 键 词： | 烟草青枯病 生物防治 拮抗真菌 |
Screening and Identification of Antagonistic Fungi in Soil Against Ralstonia solancearum |
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| LU Zheng-zheng , YANG Xian-quan , PENG Jie , JIANG Xuan-li , DING Hai-xia , PENG Li-juan.Screening and Identification of Antagonistic Fungi in Soil Against Ralstonia solancearum[J].Guizhou Agricultural Sciences,2012,40(1):86-89,175. | |
| Authors: | LU Zheng-zheng YANG Xian-quan PENG Jie JIANG Xuan-li DING Hai-xia PENG Li-juan |
| Institution: | 1,2(1.Guizhou Key Laboratory for Plant Pest Management of Mountainous Region,Guizhou University,Guiyang,Guizhou 550025;2.Agricultural College of Guizhou University,Guiyang,Guizhou 550025;3.Majiang Tobacco Bureau,Majiang,Guizhou 557600;4.Tianzhu Tobacco Bureau,Tianzhu,Guizhou 556600;5.College of Agronomy and Bio-technology,China Agricultural University,Beijing 100193,China) |
| Abstract: | Three antagonism fungus strains against R.solancearum were successfully selected and identified from 56 fungus strains isolated from the rhizosphere soil of healthy tobacco plants by the plate confrontation culture method and biological control identification method with molecular biology and morphology to effectively control tobacco bacterial wilt,improve yield and quality of tobacco and tobacco products and solve the problem of residual hazardous materials.The results showed that they were Bionectria ochroleuca,Trichoderma asperellum and Penicillium pinophilum and the antagonism effect was Bionectria ochroleuca>Trichoderma asperellum>Penicillium pinophilum. |
| Keywords: | tobacco bacterial wilt biological control antagonism fungus |
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