| 小麦品种"中国春"主要过敏原CM16基因的克隆及序列分析 |
| |
| 引用本文： | 邬玉兰,刘志刚,余汉谋,伍文琪.小麦品种"中国春"主要过敏原CM16基因的克隆及序列分析[J].麦类作物学报,2009,29(1). |
| |
| 作者姓名： | 邬玉兰 刘志刚 余汉谋 伍文琪 |
| |
| 作者单位： | 深圳大学过敏反应与免疫学研究所,广东深圳,518060 |
| |
| 基金项目： | 广东省科技重点专项基金，深圳市科技计划项目 |
| |
| 摘 要： | 为了给小麦主要过敏原CM16蛋白的重组表达和免疫活性鉴定等研究奠定基础,通过生物信息学方法设计并合成简并性引物,利用RT-PCR技术对小麦主要过敏原CM16基因进行克隆,并进行序列分析. 结果表明,克隆获得了小麦主要过敏原CM16基因.基因开放阅读框为432个碱基(包括终止密码子), 编码143个氨基酸.该序列编码的蛋白相对分子质量约为15 782,等电点为5.17.序列同源性分析发现其与国外报道的已知小麦CM16基因具有很高的同源性(同源性为99%),因此认为其系小麦过敏原基因,在GenBank数据库中的登录号为EU883599.
|
| 关 键 词： | 小麦 中国春 过敏原 基因克隆 |
Cloning and Sequence Analysis of the Major Allergen CM16 Gene in Wheat Cultivar Chinese Spring (Triticum aestivum L) |
| |
| WU Yu-lan,LIU Zhi-gang,YU Han-mou,WU Wen-qi.Cloning and Sequence Analysis of the Major Allergen CM16 Gene in Wheat Cultivar Chinese Spring (Triticum aestivum L)[J].Journal of Triticeae Crops,2009,29(1). |
| |
| Authors: | WU Yu-lan LIU Zhi-gang YU Han-mou WU Wen-qi |
| |
| Institution: | Allergy and Immunology Institute;Shenzhen University;Shenzhen;Guangdong 518060;China |
| |
| Abstract: | In order to clone CM16 gene from wheat cultivar Chinese Spring(Triticum aestivum L),CM16 gene sequences was compared and the conservative domains among these sequences were used for designing degenerate primers.The RT-PCR methods were applied to clone the full-length CM16 from Triticum aestivum L and the sequence was analyzed.Results showed that the major allergen gene CM16 of Triticum aestivum L was cloned.The cloned fragment was with 432 bp open reading frame coding for 143 amino acids.Sequence analysis s... |
| |
| Keywords: | CM16 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《麦类作物学报》浏览原始摘要信息 |
| 点击此处可从《麦类作物学报》下载免费的PDF全文 |
