| 湿地松感染松色二孢菌后过氧化物酶同工酶的测定 |
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| 引用本文： | 边银丙,周茂繁.湿地松感染松色二孢菌后过氧化物酶同工酶的测定[J].华中农业大学学报,1996,15(3):225-228. |
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| 作者姓名： | 边银丙 周茂繁 |
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| 作者单位： | 华中农业大学植保系 |
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| 摘 要： | 聚丙烯酰胺凝胶电泳分析结果表明，湿地松健康嫩茎组织过氧化物酶同工酶酶谱中有４条酶带，刺伤组织较健康组织新增加３条酶带。分别用松色二孢菌华中型（ＨＺ型）代表菌株ＪＨ－１和华南型（ＨＮ型）代表菌株ＢＹ－２接种被刺伤的湿地松嫩茎，接种弱致病力的菌株ＢＹ－２后，感病组织的过氧化物酶同工酶酶谱中比刺伤对照新增加１条酶带；接种强致病力的菌株ＪＨ－１后，感病组织的过氧化物酶同工酶酶谱中较刺伤对照新增加３条酶带。２个供试菌株在侵染寄主后导致寄主的过氧化物酶同工酶酶谱上出现差异的表现，与它们在形态特征、产孢条件和致病力等方面存在显著差异的表现相同。
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| 关 键 词： | 湿地松 松色二孢菌 过氧化物酶 同功酶 致病力 |
DETERMINATION OF THE PEROXIDASE ISOZYMES OF Pinus elliottii INFECTED BY DIFFERENT ISOLATES OF Diplodia pinea |
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| Bian Yinbing,Zhou Maofan.DETERMINATION OF THE PEROXIDASE ISOZYMES OF Pinus elliottii INFECTED BY DIFFERENT ISOLATES OF Diplodia pinea[J].Journal of Huazhong Agricultural University,1996,15(3):225-228. |
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| Authors: | Bian Yinbing Zhou Maofan |
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| Abstract: | The result of polyacrylamide gel electrophoresis showed that four bands of peroxidase isozyme existed in the young shoots of Pinus elliottii Engelm.but three more bands appeared in the pierced young shoots besides the four bands of healthy tissues. In the peroxidase isozyme profile of pierced young shoots,only one new band occured after young shoots were inoculated with hypovirulence isolate By-2 of Dipoldia pinea(Desm.)Kickx,a type HN representative isolate. However,three new bands were displayed in the peroxidase isozyme profile after P. elliottii young shoots were inoculated with virulent isolate JH-1 of D. pinea,a type HZ representative isolate.The peroxidase isozyme profiles difference of P. elliottii induced by two experimental isolates is identical with their significant differences in morphological characteristics,sporogenous conditions and virulences. |
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| Keywords: | Pinus elliottii Diplodia pinea peroxidase isozyme virulence |
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