| 大黄鱼、鮸鱼及美国红鱼线粒体DNA的Cyt b基因序列比较 |
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| 引用本文： | 李鹏飞,周永东,徐汉祥.大黄鱼、鮸鱼及美国红鱼线粒体DNA的Cyt b基因序列比较[J].南方水产,2008,4(3):43-47. |
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| 作者姓名： | 李鹏飞 周永东 徐汉祥 |
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| 作者单位： | 浙江省海洋水产研究所,浙江,舟山,316100 |
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| 基金项目： | 农业部示范项目
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浙江省科技厅资助项目 |
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| 摘 要： | 利用PCR技术对大黄鱼Pseudosciaena crocea、鮸鱼Scioenops ocellatus和美国红鱼Miichthys miiuy线粒体DNA的细胞色素b（Cytb）基因片段进行了扩增，PCR产物直接测序，分别获得大黄鱼和美国红鱼1140bp的序列，鮸鱼1125bp的序列。通过对3种鱼的cnb序列的比对分析，得出3种鱼序列的相似性为91．49％；分析了3种鱼序列的碱基组成及碱基变异情况，发现3种鱼的序列差异明显，碱基替换较多，可以将cytb基因作为种间分子鉴定或更高分类界元遗传分析的分子标记；计算了3种鱼序列的遗传距离并构建了系统树，结果显示，鮸鱼与美国红鱼的遗传距离较近。
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| 关 键 词： | 大黄鱼 鮸鱼 美国红鱼 线粒体DNA Cyt b基因 |
| 文章编号： | 1673-2227-(2008)03-0043-05 |
| 修稿时间： | 2008年3月11日 |
Sequence analysis on mitochondrial (mtDNA)Cyt b genes in Pseudosciaena crocea, Miichthys miiuy and Sciaenops ocellatus |
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| LI Pengfei,ZHOU Yongdong,XU Hanxiang.Sequence analysis on mitochondrial (mtDNA)Cyt b genes in Pseudosciaena crocea, Miichthys miiuy and Sciaenops ocellatus[J].South China Fisheries Science,2008,4(3):43-47. |
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| Authors: | LI Pengfei ZHOU Yongdong XU Hanxiang |
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| Institution: | (Marine and Fisheries Research Institute of Zhefiang Province, Zhoushan 316100, China ) |
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| Abstract: | The complete sequences of cytochrome b （ Cyt b ） genes in Pseudosciaena crocea and Scioenops ocellatus, and the partial sequence of cytochrome b （Cyt b） gene in Miichthys miiuy were amplified using PCR technology. The PCR products were sequenced directly. 1 140 bp fragments of Cyt b genes in P. crocea and S. ocellatus, and 1 125 bp fragment of Cyt b gene in M. miiuy were obtained. Comparing obtained sequences, the comparability similarity of three sequences was 91.49%. Information of the base composition and the mutation sites were obtained. The difference of three sequences was obvious. The results contributed not only genetic data to economic utilization of three species but also scientific basis to analysis of genetic diversity in China Sea. Moreover, molecular phylogcnetic tree of the Cyt b DNA sequences in the three species was reconstructed with the NJ method based on the inherited distance and the result showed that the inherited distance between the M. miiuy and the S. ocellatus was small. |
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| Keywords: | Preudosciaena crocea Miichthys miiuy Sciaenops ocellatus mitochondrial DNA （mtDNA） Cyt b gene |
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