| 生物絮凝剂生产菌的分离与鉴定及其絮凝特性分析(摘要)(英文) |
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| 引用本文： | 杨金水,马龙,刘伟杰,李宝珍,袁红莉.生物絮凝剂生产菌的分离与鉴定及其絮凝特性分析(摘要)(英文)[J].Agricultural Science&Technology,2010(8). |
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| 作者姓名： | 杨金水 马龙 刘伟杰 李宝珍 袁红莉 |
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| 作者单位： | 中国农业大学生物学院农业部农业微生物资源及其应用重点实验室; |
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| 基金项目： | Supported by Special Project of Graduate Research and Innovation(2009); National Natural Science Foundation of China (30700010); Special Fund for Agro-scientific Research in the Public Interest(200803033)~~ |
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| 摘 要： | 目的]对分离自土壤样品中的一株微生物絮凝剂产生菌W5进行系统鉴定并分析其絮凝特性。方法]以细菌的16SrDNA通用引物对其基因组进行PCR扩增,并采用单因素法分析培养基的pH值、碳源和氮源对絮凝活性的影响,采用红外光谱法进行功能基团的分析。结果]同源性比对结果表明菌株W5与Hymenobacter gelipurpurascens具有98.34%的同源性,为革兰氏阴性,长杆菌。菌株W5生长发酵培养基最佳条件为pH值7.04、葡萄糖为碳源、酵母粉为氮源。对菌株W5所产的微生物絮凝剂(MBF-W5)进行提取纯化,所得产物微红色絮状物。红外分析结果表明,MBF-W5中主要的功能基团为羟基、羧基和氨基。结论]在絮凝剂用量为150～500μl,温度为5～65℃,pH值为2～10的范围内均具有85%以上的絮凝活性。
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| 关 键 词： | Hymenobacter gelipurpurascens W5 微生物絮凝剂 MBF-W5 絮凝活性 |
Isolation and Identification of Bioflocculant Producing Stain and Analysis on Its Flocculating Characteristics |
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| YANG Jin-shui,MA Long,LIU Wei-jie,LI Bao-zhen,YUAN Hong-li College of Biological Science,China Agricultural University,Key Laboratory of Agro-Microbial Resource , Application of Ministry of Agriculture,Beijing.Isolation and Identification of Bioflocculant Producing Stain and Analysis on Its Flocculating Characteristics[J].农业科学与技术(英文版),2010(8). |
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| Authors: | YANG Jin-shui MA Long LIU Wei-jie LI Bao-zhen YUAN Hong-li College of Biological Science China Agricultural University Key Laboratory of Agro-Microbial Resource Application of Ministry of Agriculture Beijing |
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| Institution: | YANG Jin-shui,MA Long,LIU Wei-jie,LI Bao-zhen,YUAN Hong-li College of Biological Science,China Agricultural University,Key Laboratory of Agro-Microbial Resource and Application of Ministry of Agriculture,Beijing 10019 |
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| Abstract: | Objective] The aim was to indentify a bioflocculatnt producing strain W5 isolated from soil samples and analyze its flocculating characteristics. Method] The universal primers of bacterial 16S rDNA were used for PCR amplification of its genome; effects of the medium pH,carbon and nitrogen sources on its flocculating activity were analyzed by using single factor method; and infrared spectroscopy was used for functional groups analysis. Result] The results of homology comparison showed that strain W5 belon... |
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| Keywords: | Hymenobacter gelipurpurascens W5 Microbe flocculant Flocculating activity |
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