| 水果型番木瓜组织培养的研究(英文) |
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| 引用本文： | 谢恩倍,欧善生,姚军,石梅娟,梁盛,朱国兵,闵华,赵广存.水果型番木瓜组织培养的研究(英文)[J].Agricultural Science&Technology,2010(7). |
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| 作者姓名： | 谢恩倍 欧善生 姚军 石梅娟 梁盛 朱国兵 闵华 赵广存 |
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| 作者单位： | 广西农业职业技术学院; |
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| 基金项目： | Support by Guangxi Vocational Technical College of Agriculture Scientific Research and Technological Development Projects of China(Agriculture Vocational Department B070210)~~ |
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| 摘 要： | 目的]研究水果型番木瓜的组织培养。方法]以经预处理的种子为试材,通过MS基本培养基附加不同浓度的6-BA和IBA,对水果型番木瓜组培快繁技术进行了研究。结果]无菌条件下用75%酒精均匀擦拭水果型番木瓜果皮表面,取出种子,用无菌水冲洗3次是较好的灭菌方法,种子污染率仅为2.52%;水果型番木瓜种子经1000mg/LGA3+1mg/L6-BA(等体积混合)浸泡18h后接种于MS培养基中培养,种子发芽率、胚芽长度、胚根长度和苗高达到较高值,分别为68.42%、2.25、0.80和1.52cm,幼苗整体生长较好;继代增殖培养基为MS+0.5mg/L6-BA+0.1mg/LIBA时增殖系数最高(7.92),幼苗长势较好;组培苗玻璃化随着光照强度的增强而呈减弱趋势,3000lx处理下玻璃化苗比率最低(3.21%)。结论]该研究为水果型番木瓜的组培快繁研究提供了参考。
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| 关 键 词： | 番木瓜 种子 GA3 6-BA 组培 |
Tissue Culture of Mini-Papaya |
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| XIE En-bei,OU Shan-sheng,YAO Jun,SHI Mei-juan,LIANG Sheng,ZHU Guo-bing,MIN Hua,ZHAO Guang-cun Guangxi Vocational Technical College of Agriculture,Nanning.Tissue Culture of Mini-Papaya[J].农业科学与技术(英文版),2010(7). |
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| Authors: | XIE En-bei OU Shan-sheng YAO Jun SHI Mei-juan LIANG Sheng ZHU Guo-bing MIN Hua ZHAO Guang-cun Guangxi Vocational Technical College of Agriculture Nanning |
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| Institution: | XIE En-bei,OU Shan-sheng,YAO Jun,SHI Mei-juan,LIANG Sheng,ZHU Guo-bing,MIN Hua,ZHAO Guang-cun Guangxi Vocational Technical College of Agriculture,Nanning 530007 |
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| Abstract: | Objective] The aim was to study the tissue culture of mini-Papaya.Method] The pretreatment seeds of mini-Papaya were cultured in the MS medium containing 6-BA and IBA of different densities for rapid propagation.Result] In the condition of aseptic strain,the surface of mini-Papaya peel was uniformly wiped by 75% alcohol,then seeds were removed and washed by aseptic water for 3 times,which was the best sterilization method,and the pollution rate of seed was only 2.52%.After seeds which had been soaked by ... |
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| Keywords: | Papaya Seed GA3 6-BA Tissue culture |
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