Structural characterization of potato protease inhibitor I (Cv. Bintje) after expression in Pichia pastoris |
| |
| Authors: | van den Broek Lambertus A M Pouvreau Laurice Lommerse Gijs Schipper Bert Van Koningsveld Gerrit A Gruppen Harry |
| |
| Institution: | Centre for Protein Technology TNO-WU, P.O. Box 8129, 6700 EV Wageningen, The Netherlands. |
| |
| Abstract: | In the present study the structural properties of potato protease inhibitor 1 (PI-1) were studied as a function of temperature to elucidate its precipitation mechanism upon heating. A cDNA coding for PI-1 from cv. Bintje was cloned and expressed in Pichia pastoris. Using the recombinant PI-1 it was suggested that PI-1 behaves as a hexameric protein rather than as a pentamer, as previously proposed in the literature. The recombinant protein seems either to have a predominantly unordered structure or to belong to the beta-II proteins. Differential scanning calorimetry analysis of PI-1 revealed that its thermal unfolding occurs via one endothermic transition in which the hexameric PI-1 probably unfolds, having a dimer instead of a monomer as cooperative unit. The transition temperature for the recombinant PI-1 was 88 degrees C. Similar results were obtained for a partially purified pool of native PI-1 from cv. Bintje. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
