| 猪附红细胞体巢式PCR诊断方法的建立及初步应用 |
| |
| 引用本文： | 郑秀红,邢莹,贾立军,薛书江,甄立家,张守发.猪附红细胞体巢式PCR诊断方法的建立及初步应用[J].中国畜牧兽医,2011,38(1):194-197. |
| |
| 作者姓名： | 郑秀红 邢莹 贾立军 薛书江 甄立家 张守发 |
| |
| 作者单位： | （1.延边大学农学院动物医学系，吉林延吉 133002；2.吉林省东丰县大阳镇畜牧兽医站，吉林东丰 136309） |
| |
| 基金项目： | 国家自然科学基金项目(30860203); 公益性行业(农业)科研专项项目(200903036-13) |
| |
| 摘 要： | 为建立一种更加准确、敏感的猪附红细胞体检测方法，本试验根据猪附红细胞体特异的DNA序列(AJ504999)设计2对巢式PCR引物，建立了巢式PCR检测方法。筛选该检测方法的最佳反应条件，并进行了特异性、敏感性及临床样本检测试验。结果表明，建立的巢式PCR对猪附红细胞体基因组DNA能扩增出特异性片段，而对弓形虫、链球菌、牛瑟氏泰勒虫、牛附红细胞体基因组DNA扩增反应结果均为阴性；DNA最低检测量为0.124 fg/μL；通过对55份临床样本的检测，该巢式PCR较常规PCR的阳性检出率高23.7%。本试验为猪附红细胞体病的诊断提供了一种更为特异、敏感的检测技术。
|
| 关 键 词： | 猪附红细胞体 巢式PCR 建立 应用 |
| 收稿时间： | 2010-07-26 |
Establishment and Application of Nested PCR Assay for Detection of Eperythrozoon suis |
| |
| ZHENG Xiu-hong,XING Ying,JIA Li-jun,XUE Shu-jiang,ZHEN Li-jia,ZHANG Shou-fa.Establishment and Application of Nested PCR Assay for Detection of Eperythrozoon suis[J].China Animal Husbandry & Veterinary Medicine,2011,38(1):194-197. |
| |
| Authors: | ZHENG Xiu-hong XING Ying JIA Li-jun XUE Shu-jiang ZHEN Li-jia ZHANG Shou-fa |
| |
| Institution: | （1. Department of Veterinary Medicine,College of Agriculture,Yanji University,Jilin 133002, China; 2. Dayang Prologue Station, Dongfeng County,Jilin Province,Dongfeng 136309, China） |
| |
| Abstract: | To establish a more exact and sensitive method to detect Eperythrozoon suis,two pairs of nested PCR primer were designed according to Eperythrozoon suis DNA sequence(AJ504999)and nested PCR assay was established.The optimum reaction conditions were screened,and sensitive,special and clinical samples tests were carried out.The results showed that Eperythrozoon suis genome DNA could be amplified by nested PCR,and Toxoplasma gondii,Streptococcus,Theileria sergenti and Eperythrozoon wenyonii genomic DNA could n... |
| |
| Keywords: | Eperythrozoon suis nested PCR establishment application |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
