| 雷蒙德氏棉Argonaut基因家族的生物信息学预测与分析 |
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| 引用本文： | 刘国元,吴嫚,于霁雯,裴文锋,喻树迅.雷蒙德氏棉Argonaut基因家族的生物信息学预测与分析[J].棉花学报,2014,26(5):411-419. |
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| 作者姓名： | 刘国元 吴嫚 于霁雯 裴文锋 喻树迅 |
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| 作者单位： | 中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南安阳,455000 |
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| 基金项目： | 国家自然科学基金，国家973计划，国家863计划 |
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| 摘 要： | Argonaute(AGO)蛋白是参与小RNA调控通路的重要元件,在生命体的生长发育过程中发挥着重要作用.本研究利用AGO蛋白的保守域在雷蒙德氏棉基因组中进行同源比对,鉴定出15个成员,并对其进行进化关系、染色体定位、基因结构、保守域、时空表达模式等分析.结果表明:该家族成员基因长度从3335到9086bp不等,其中11个基因被定位到7条染色体上;该家族成员可分为3个亚族,包含5个小组,各亚族内、小组间成员也存在很高的保守性;发现每个基因都有不同的时空表达模式,且各小组内成员之间存在协同和互补2种表达模式,找到在花器官特异表达的基因1个,胚珠中特异表达的基因3个.本文首次对棉花中AGO基因家族进行了鉴定和生物信息学分析,为进一步研究AGO基因家族在棉花中的功能奠定了基础.
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| 关 键 词： | 雷蒙德氏棉 AGO基因家族 基因结构 表达模式 |
| 收稿时间： | 2014-03-03 |
Genome-wide Identification of Argonaute Gene Family in Gossypium raimondii Using Bioinformatic Analysis |
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| Liu Guoyuan,Wu Man,Yu Jiwen,Pei Wenfeng,Yu Shuxun.Genome-wide Identification of Argonaute Gene Family in Gossypium raimondii Using Bioinformatic Analysis[J].Cotton Science,2014,26(5):411-419. |
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| Authors: | Liu Guoyuan Wu Man Yu Jiwen Pei Wenfeng Yu Shuxun |
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| Institution: | Institute of Cotton Research of Chinese Academy of Agriculture Sciences/State Key Laboratory of Cotton Biology, Anyang, Henan 455000, China |
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| Abstract: | Argonaute(AGO) proteins play a very important role in small RNA-regulated pathways related to growth and development, defense against virus, histogenesis, cell proliferation, and apoptosis. We identified 15 AGO family members in the Gossypium raimondii(G. raimondii) genome, and used analyses of phylogeneties, gene structure, domains, and motifs to show that this family is highly conserved. The AGO gene family members varied from 3335 to 9086 bp in length, and 11 genes were located in seven chromosomes. The family can be divided into three groups. Each gene has a specific expression pattern in different tissues, with one gene expressed in flower, three genes in ovule. This identification and bioinformatics analysis lays the foundations for the future study of AGO gene family in G. raimondii. |
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| Keywords: | G raimondii AGO gene family gene structure expression pattern |
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