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| 昆虫病毒重组增效蛋白的广谱增效活性 | |
| 引用本文： | 袁哲明,孟小林,刘树生.昆虫病毒重组增效蛋白的广谱增效活性[J].中国生物防治,2004,20(1):31-33. |
| 作者姓名： | 袁哲明 孟小林 刘树生 |
| 作者单位： | 1. 湖南农业大学植物保护学院,长沙,410128 2. 武汉大学病毒学研究所 3. 浙江大学应用昆虫学研究所 |
| 基金项目： | 国家自然科学基金 (395 70 0 34和 30 10 0 12 2 )，湖南省自然科学基金 (0 0JJY2 0 18) |
| 摘 要： | 室内生物测定表明 ,大肠杆菌表达的粉纹夜蛾颗粒体病毒重组增效蛋白P96可显著或极显著提高棉铃虫核型多角体病毒、苏云金杆菌和阿维菌素对棉铃虫幼虫的致死率 ,分别提高1 6 9.78%、73.90 %和 36 .0 4 % ;对苜蓿丫纹夜蛾核型多角体病毒感染甜菜夜蛾幼虫也有明显增效作用。Na2 CO3 溶解的P96对棉铃虫初孵幼虫有一定致死作用。 |
| 关 键 词： | 重组增效蛋白 广谱增效剂 生物测定 |
| 文章编号： | 1005-9261(2004)01-0031-03 |
| 修稿时间： | 2002年11月1日 |
Wide-spectrum Synergy of Recombinant Enhancin from Insect Baculovirus Expressed in Escherichia coli |
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| YUAN Zhe-ming,MENG Xiao-lin,LIU Shu-sheng.Wide-spectrum Synergy of Recombinant Enhancin from Insect Baculovirus Expressed in Escherichia coli[J].Chinese Journal of Biological Control,2004,20(1):31-33. | |
| Authors: | YUAN Zhe-ming MENG Xiao-lin LIU Shu-sheng |
| Abstract: | A 2.5kb fragment of enhancin gene from Trichoplusia ni granulovirus was inserted into vector pQE-31 and expressed successfully in Escherichia coli M15 (pREP4) in the previous study. A series of bioassays were conducted to characterize the wide-spectrum synergy of the expression product named as P96 in this paper. After combination of P96, mortalities caused by Helicoverpa armigera nuclear polyhedrosis virus (HaNPV), Bacillus thuringiensis and avermectin, against the larvae of H.armigera were increased significantly by 169.78 %, 73.90% and 36.04%, respectively. P96 was also shown to increase the susceptibility of Spodoptera exigua to Autographa californica NPV. Cumulative corrected mortality caused by P96 dissolved in Na 2CO 3 solution to the 1st instar larvae of H.armigera was 15.91% on the 5th day after treated. As a wide-spectrum synergist, P96 may therefore play an important role in integrated pests management. |
| Keywords: | recombinant enhancin wide-spectrum synergist bioassay |
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