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| 甲型H1N1与季节性H1N1流感病毒检测基因芯片的制备与初步应用 | |
| 引用本文： | 孙珊珊,沈国顺,田明尧,鲁会军,李昌,李霄,颜雯,谭磊,李洋,韩佳丽,金宁一.甲型H1N1与季节性H1N1流感病毒检测基因芯片的制备与初步应用[J].中国兽医学报,2010,30(5). |
| 作者姓名： | 孙珊珊 沈国顺 田明尧 鲁会军 李昌 李霄 颜雯 谭磊 李洋 韩佳丽 金宁一 |
| 作者单位： | 1. 沈阳农业大学畜牧兽医学院,辽宁沈阳110161;军事医学科学院军事兽医研究所,吉林长春,130062 2. 沈阳农业大学畜牧兽医学院,辽宁沈阳,110161 3. 军事医学科学院军事兽医研究所,吉林长春,130062;吉林大学畜牧兽医学院,吉林长春130062 4. 军事医学科学院军事兽医研究所,吉林长春,130062 5. 军事医学科学院军事兽医研究所,吉林长春,130062;吉林大学第一医院呼吸科,吉林长春130021 |
| 基金项目： | 国家科技重大专项基金资助项目(2009ZX10004-103); 国家科技支撑计划基金资助项目(2010BAD04B02); 吉林省科技发展计划项目基金资助项目(20090486) |
| 摘 要： | 建立DNA微列阵技术检测甲型H1N1和季节性H1N1流感病毒的方法,进而探讨该方法用于检测临床标本的可行性。通过生物医学数据库甲型H1N1流感病毒及季节性H1N1亚型流感HA与NA基因进行检索和筛选,应用分子生物学软件,进行序列分析、引物及探针设计,并进行验证。试验所设计的探针可以对甲型H1N1流感与季节性H1N1流感病毒进行区分,用该方法检测了长春市CDC送检的6份疑似甲型H1N1流感病毒临床病例,4份阳性,检测结果同实时荧光定量PCR方法一致。结果表明,利用本研究建立的DNA微列阵技术检测甲型H1N1流感病毒方法,特异性和敏感性强,可作为甲型H1N1流感病毒临床标本检测方法。 |
| 关 键 词： | 甲型H1N1流感病毒 核酸探针 杂交 检测 |
Preparation and application of diagnostic gene chip for novel A (H1N1) influenza and H1N1 seasonal influenza virus |
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| SUN Shan-shan,SHEN Guo-shun,TIAN Ming-yao,LU Hui-jun,LI Chang,LI Xiao,YAN Wen,TAN Lei,LI Yang,HAN Jia-li,JIN Ning-yi.Preparation and application of diagnostic gene chip for novel A (H1N1) influenza and H1N1 seasonal influenza virus[J].Chinese Journal of Veterinary Science,2010,30(5). | |
| Authors: | SUN Shan-shan SHEN Guo-shun TIAN Ming-yao LU Hui-jun LI Chang LI Xiao YAN Wen TAN Lei LI Yang HAN Jia-li JIN Ning-yi |
| Institution: | SUN Shan-shan1,2,SHEN Guo-shun1,TIAN Ming-yao2,3,LU Hui-jun2,LI Chang2,LI Xiao2,YAN Wen2,TAN Lei2,LI Yang2,4,HAN Jia-li1,JIN Ning-yi2 (1.College of Animal Science , Veterinary Medicine,Shenyang Agriculture University,Shenyang 110161,China,2.Institute of Military Veterinary,Academy of Military Medical Science of PLA,Changchun 130062,3.College of Animal Science , Veterinary Medicine,Jilin University,4.Department of Respiration,the First Clinical Hospital of Jilin Univers... |
| Abstract: | To establish DNA microarray technology for the detection of novel A (H1N1) influenza and H1N1 seasonal influenza virus,respectively,and then discussed the methods to test the feasibility of clinical specimens.The HA gene and NA gene of current influenza virus H1N1 and seasonal H1N1 were indexed and screened through the biomedical database.Sequence analysis and probe primer design were tested by applying molecular biology software.The designed probes can identify the novel A (H1N1) influenza and H1N1 seasona... |
| Keywords: | novel A (H1N1) Influenza virus microprobe hybridization detection |
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