| 不同芍药甘草配伍用于溃疡性结肠炎的治疗作用及机制研究 |
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| 作者姓名： | 陈晓峰 谢君 |
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| 作者单位： | 张家港市中医医院药剂科, 江苏 张家港 215600,张家港市中医医院药剂科, 江苏 张家港 215600 |
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| 基金项目： | 江苏省自然科学基金计划（815269）。 |
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| 摘 要： | 目的 研究不同芍药甘草配伍用于溃疡性结肠炎（ulcerative colitis，UC）的治疗作用及机制。方法 纳入90只实验小鼠作为研究对象，随机抽签分为观察1组、观察2组及对照组，各30只。观察1组和观察2组采用乙酸法建立UC小鼠模型，分别以3：1和1：1比例配伍芍药甘草，以芍药甘草汤进行干预。对照组正常饲养，未行其他干预。记录3组小鼠胸腺指数、脾脏指数及结肠组织核因子-kBp65（NF-kBp65），比较观察1组和观察2组小鼠结肠收缩频率、扭体次数、炎性细胞数、炎性细胞面积、平均光密度及平均黑度总和。结果 观察1组给药30 min和1 h后结肠收缩频率和扭体次数显著低于观察2组和给药前（P<0.05）。观察1组胸腺和脾脏指数均高于观察2组，结肠组织NF-kBp65低于观察2组（P<0.05）。观察1组炎性细胞数、炎性细胞面积、平均光密度及平均黑度总和均显著低于观察2组（P<0.05）。结论 3：1比例配伍芍药甘草较1：1比例配伍的芍药甘草汤用于治疗UC，其止痛止泻效果更佳，这可能与控制炎性浸润、刺激免疫器官分泌免疫细胞，抑制NF-kBp65激活有关。
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| 关 键 词： | 芍药 甘草 溃疡性结肠炎 |
| 收稿时间： | 2016/12/26 0:00:00 |
Study on Therapeutic Effect and Mechanism of Different Compatibility of Peony and Licorice on Ulcerative Colitis |
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| Authors: | CHEN Xiaofeng and XIE Jun |
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| Institution: | Department of pharmacy, Zhangjiagang Traditional Chinese Medicine Hospital, Zhangjiagang, Jiangsu 215600, China and Department of pharmacy, Zhangjiagang Traditional Chinese Medicine Hospital, Zhangjiagang, Jiangsu 215600, China |
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| Abstract: | Objective To study the therapeutic effect and mechanism of different compatibility of peony and licorice on ulcerative colitis (UC). Methods The 90 experimental mice were randomly divided into the No. 1 observation group, No. 2 observation group, and control group, 30 mice in each group. The UC model mice of the No. 1 observation group and No. 2 observation group were built by the acetic acid method, intervened with interved peony Licorice decoction, the compatibility of peony and licorice with 3:1 and 1:1 in the two groups. The control group was fed normally without other interventions. The thymus spleen index and colonic tissue nuclear factor-kBp65 (NF-kBp65) were recorded in three groups, the colonic contraction frequency, writhing, inflammatory cell number, size, density and average total blackness between No. 1 observation group and No. 2 observation group were observed. Results The colonic contraction frequency and writhing frequency in No. 1 observation group after giving medicine for 30 minutes and 1 hour were lower than the control group and before drug administration (P<0.05). The thymus and spleen indexes in No.1 observation group were higher than that in the No.2 observation group, and colon tissue NF-kBp65 was lower than the No.2 observation group (P<0.05). The number of inflammatory cells, inflammatory cell area, the average optical density and the average total blackness of the No.1 observation group were significantly lower than those in No.2 observation group (P<0.05). Conclusion The 3:1 proportion of peony and licorice are more effective in improving the pain and diarrhea than 1:1 ratio in the treatment for UC. This may be related to controlling inflammatory infiltration, stimulating immune organ secreting immune cells and inhibiting NF-kB activation. |
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| Keywords: | peony licorice ulcerative colitis |
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