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| 生物频谱对牛胚胎体外生产效率的影响 | |
| 引用本文： | 曾申明,朱士恩,张忠诚,安晓荣,陈永福,陈锟,周林.生物频谱对牛胚胎体外生产效率的影响[J].中国兽医学报,2000,20(2):188-191. |
| 作者姓名： | 曾申明 朱士恩 张忠诚 安晓荣 陈永福 陈锟 周林 |
| 作者单位： | 1. 中国农业大学,动物科技学院,北京,100094 2. 中国农业大学,生物学院,北京,100094 3. 北京周林频谱公司,北京,100083 |
| 基金项目： | 国家“八六三”计划资助项目! ( Z2 1-0 1-0 1) |
| 摘 要： | 利用可调式周林生物频谱发生器对牛卵母细胞体外成熟、体外受精和体外培养过程实施辐射，以提高牛胚胎体外生产效率。频谱发生器与培养材料的距离为５ｃｍ，辐射强度根据辐射温度加以调节。结果表明，辐射温度控制在３９℃时，入孵卵母细胞的卵裂率（６３％）与对照组６１（％）无显著差异（Ｐ〉０．０５），而囊胚发育率（３９％）显著高于对照组（１９％）（Ｐ〈０．０５）；当辐射温度达到４０℃时，试验组的卵裂率仅为１４％，囊 |
| 关 键 词： | 生物频谱 体外受精 玻璃化冷冻 牛 |
| 修稿时间： | 1999-01-25 |
Effects of Biofrequency Spectrum Irradiation on Efficiency of Bovine Embryo Production In vitro |
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| ZENG Shen-ming,ZHU Shi-en,ZHANG Zhong-cheng,AN Xiao-rong,CHEN Yong-fu,CHEN Kun,ZHOU Lin.Effects of Biofrequency Spectrum Irradiation on Efficiency of Bovine Embryo Production In vitro[J].Chinese Journal of Veterinary Science,2000,20(2):188-191. | |
| Authors: | ZENG Shen-ming ZHU Shi-en ZHANG Zhong-cheng AN Xiao-rong CHEN Yong-fu CHEN Kun ZHOU Lin |
| Abstract: | The effects of biofrequency spectrum(BFS) irradia tion during in vitro fertilization on bovine embryo production i n vitro were studied. The distance from the BFS apparatus to the oocytes or em bryos was 5 cm dur ing irradiation, and the irradiation intensity was adjusted so as to deep the ir r adiation temperature at 39℃ or 40℃. The results showed that the rate of cleava ges existed no significant difference between the irradiation and the control gr oup when the irradiating temperature was kept at 39℃(P>0.05), however, the blastocysts rate in the irradiation group was significantly higher than that in the control (P<0.05) . When the irradiation temperature was 40℃, both rates of cleavages and blastoc ysts in the irradiation group were greatly significantly lower than that in the control (P <0.01). The early blastocysts from in vitro production with an d without BFS irradiation were cryopreserved by vitrification in EFS40 solution respectively, the developmental rate of the blastocysts with BFS irradiation was significantly higher than that of the without BFS irradiation (P<0.05). |
| Keywords: | biofrequency in vitro fertilization vitrification bovine |
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