|
|||||
|
|
| 不同品质粗饲料日粮对瘤胃发酵及主要纤维分解菌的影响 | |
| 引用本文： | 刘大程,卢德勋,侯先志,高民,孙海洲.不同品质粗饲料日粮对瘤胃发酵及主要纤维分解菌的影响[J].中国农业科学,2008,41(4):1199-1206. |
| 作者姓名： | 刘大程 卢德勋 侯先志 高民 孙海洲 |
| 作者单位： | 1. 内蒙古农业大学动物科学与医学学院,呼和浩特,010018;内蒙古畜牧科学院,呼和浩特,010030 2. 内蒙古畜牧科学院,呼和浩特,010030 3. 内蒙古农业大学动物科学与医学学院,呼和浩特,010018 |
| 摘 要： | 【目的】研究不同品质粗饲料组成的混合日粮对绵羊瘤胃发酵功能和3种主要纤维分解菌的影响。【方法】以粗饲料分级指数GI为指标对粗饲料品质进行调配，在精粗比为2﹕8条件下配制3种混合日粮，其中含高GI粗饲料日粮组（H组，GI值＝2.89）、含中GI粗饲料日粮组（M组，GI值＝1.73）和含低GI粗饲料日粮组（L组，GI值＝0.65）。利用16S rRNA特异性寡核苷酸探针杂交法测定3种纤维分解菌的数量变化。【结果】三组试验动物瘤胃液pH维持在正常范围，各组间差异不显著（P＞0.05），但H组pH变化更为平缓。随着GI指数的升高，瘤胃液NH3-N浓度呈升高势态，H组显著高于L组（P＜0.05），乙酸浓度有降低的趋势，而丙酸浓度则有上升的趋势，乙酸/丙酸出现降低趋势，各试验组TVFA浓度的平均值差异不显著（P＞0.05）。黄化瘤胃球菌在绵羊瘤胃中相对含量的总平均值为1.18%，白色瘤胃球菌的相对含量为2.44%，琥珀酸拟杆菌的含量为10.07%。【结论】不同品质粗饲料日粮显著影响绵羊瘤胃产琥珀酸丝状杆菌的数量，随着粗饲料品质的升高，该菌的数量有增加的趋势；不同品质粗饲料日粮改变了瘤胃的发酵方式和发酵功能。 |
| 关 键 词： | 粗饲料品质 瘤胃发酵 寡核苷酸探针 纤维分解菌 瘤胃细菌 |
| 收稿时间： | 2007-1-29 |
| 修稿时间： | 2007年1月29日 |
Effect of Dietary Forage of Different Qualities on Rumen Fermentation and Predominant Fibrolytic Bacterial Populations |
|
| LIU Da-cheng,LU De-xun,HOU Xian-zhi,GAO Min,SUN Hai-zhou.Effect of Dietary Forage of Different Qualities on Rumen Fermentation and Predominant Fibrolytic Bacterial Populations[J].Scientia Agricultura Sinica,2008,41(4):1199-1206. | |
| Authors: | LIU Da-cheng LU De-xun HOU Xian-zhi GAO Min SUN Hai-zhou |
| Abstract: | On the same dietary ratio of forage to concentrate（R/C）of 8:2, experimental animals were divided into H group(GI 2.89)、M group(GI 1.73) and L group(GI 0.65) according to Grading Index(GI) of forage, to study the effect of different quality of forage on rumen fermentation and quantity of predominant fibrolytic bacterial population. The results showed that the pH values of the animals in three groups were all in normal range, the interclass differences were not significant（p>0.05）,and the change of H group’s pH values was more mild. The ruminal NH3-N concentration display ascendant tendency,in the animals of H group was more significant than L group（p<0.05）,and the ruminal concentration of acetic acid had reducing tendency, the concentration of propanoic acid had ascendant tendency, the acetate to propionate ratio had reducing tendency, the mean value’s differences of the ruminal concentration of total VFA concentration among three groups were not significant（p>0.05）. By hybridization analysis, the relative amount of R.flaveciens﹑R.albus and B.succinogenes in the rumen of the sheep were 1.18％、2.44％and 10.07％, respectively. |
| Keywords: | the quality of forage rumen fermentation oligonucleotide probe cellulolytic bacteria ruminal bacteria |
| 本文献已被 维普 万方数据 等数据库收录！ | |
| 点击此处可从《中国农业科学》浏览原始摘要信息 | |
| 点击此处可从《中国农业科学》下载免费的PDF全文 | |