| 金属离子对红白锦鲤肠及肝胰脏蛋白酶活性的影响（摘要）（英文） |
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| 引用本文： | 郭永军,魏东,白东清,闫珊珊,吴旋,宁博,陆建权.金属离子对红白锦鲤肠及肝胰脏蛋白酶活性的影响（摘要）（英文）[J].农业科学与技术,2010,11(3):104-106,122. |
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| 作者姓名： | 郭永军 魏东 白东清 闫珊珊 吴旋 宁博 陆建权 |
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| 作者单位： | 天津农学院水产科学系天津市水产生态及养殖重点实验室,天津300384 |
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| 摘 要： | 目的]研究金属离子对红白锦鲤消化组织蛋白酶活性的影响。方法]以酶学分析方法研究4种金属离子（Na＋、K＋、Ca2 ＋、Mg2 ＋分别来源于NaCl、KCl、CaCl2.2H2O、MgCl2.6H2O,浓度梯度均设置为25、50、75、100、125、150 mmol/L）对红白锦鲤肝胰脏、前肠、中肠、后肠蛋白酶活性的影响。蛋白酶活性的测定采用福林-酚显色法,用Thermo紫外分光光度计,根据OD680计算蛋白酶活性,以1 min水解酪蛋白产生1μg酪氨酸为一个酶活力单位,记为μg/（min.g）。结果]在设定的浓度范围内（25 ～150 mmol/L）,4种金属离子对蛋白酶活性影响不同。K＋对肝胰脏和后肠的蛋白酶活性有不同程度的促进作用,其中K＋对中肠表现为低浓度促进,高浓度抑制的作用,对前肠蛋白酶表现为抑制作用;Na＋对肝胰脏、前肠和后肠的蛋白酶表现为不同程度促进作用,对中肠表现为抑制作用。Mg2 ＋、Ca2 ＋对肠及肝胰脏的蛋白酶均表现为不同程度的抑制作用。试验所选用的K＋、Na＋、Mg2 ＋、Ca2 ＋均属矿物元素,其中添加浓度25 mmol/LK＋对锦鲤肠蛋白酶活性促进作用最大;浓度150 mmol/L Na＋显著提高后肠和肝胰脏蛋白酶活性。这些浓度可作为饲料添加剂中的参考用量。Mg2 ＋、Ca2 ＋对锦鲤各消化器官（中肠除外）的抑制作用随浓度的增加蛋白酶活性降低,但考虑该元素系水产动物生长发育所必需,因此可选用低浓度或螯合物进行添加,以提高酶的生物学效价,提高饲料中蛋白的利用率,降低饵料系数,节约蛋白。结论]该研究可为红白锦鲤消化生理的研究以及配合饲料的开发、优化提供基础数据和理论依据。
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| 关 键 词： | 红白锦鲤（Cyprinus carpio L.） 金属离子 蛋白酶活性 |
Effect of Metal Ions on Protease Activities in the Intestines and Hepatopancreas of Red-white Ornamental Carp (Cyprinus carpio L) |
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| GUO Yong-jun,WEI Dong,BAI Dong-qing,YAN Shan-shan,WU Xuan,NING Bo,LU Jian-quan.Effect of Metal Ions on Protease Activities in the Intestines and Hepatopancreas of Red-white Ornamental Carp (Cyprinus carpio L)[J].Agricultural Science ＆ Technology,2010,11(3):104-106,122. |
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| Authors: | GUO Yong-jun WEI Dong BAI Dong-qing YAN Shan-shan WU Xuan NING Bo LU Jian-quan |
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| Institution: | Tianjin Key Laboratory of AQUA-Ecology and Aquaculture,Department of Fishery Science,Tianjin Agricultural University,Tianjin 300384 |
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| Abstract: | Objective] The aim of this study was to study effects of metal ions on the protease activities in digestive tissues and gland of red-white ornamental carp（Cyprinus carpio L）.Method] Effects of four kinds of metal ions （K＋,Na＋,Mg2＋ and Ca2＋） on protease activities in hepatopancreas,foregut,midgut,hindgut of red-white ornamental carp were studied by enzyme analysis method.Result] Effects of four kinds of metal ions on protease activities of red-white ornamental carp were different in the range of experimental concentration from 25 mmol/L to 150 mmol/L.K＋ could promote protease activities in hepatopancreas and hindgut at different levels.Especially,K＋ had the promoting effect at low-concentration level,but the inhibitory effect at high-concentration level in midgut and the inhibitory effect in foregut.Na＋ had the promoting effect on protease activities in hepatopancreas,foregut and hindgut at different levels,but the inhibitory effect in midgut.Mg2＋ and Ca2＋ had the inhibitory effect on protease activities in intestinal and hepatopancreas at different levels.Conclusion] This study provides basic data and theoretical foundation for researches on the digestive physiology of red-white ornamental carp or the development and optimization of compound feed. |
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| Keywords: | Red-white ornamental carp Metal ions Protease activities |
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