| 梨‘中矮1号’LUE1基因的克隆及功能分析 |
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| 引用本文： | 郝宁宁,段续伟,欧春青,陈秋菊,王斐,姜淑苓,马力.梨‘中矮1号’LUE1基因的克隆及功能分析[J].园艺学报,2017,44(5):839-849. |
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| 作者姓名： | 郝宁宁 段续伟 欧春青 陈秋菊 王斐 姜淑苓 马力 |
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| 作者单位： | 1中国农业科学院果树研究所，农业部园艺作物种质资源利用重点实验室，辽宁兴城 125100；2北京市农林科学院林业果树研究所，北京 100093 |
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| 基金项目： | 中国农业科学院科技创新工程项目，国家科技支撑计划项目(2013D02B01;2013BAD01B04-23)，辽宁省果树产业技术体系专项(LNGSCYTX-15-X)，农业公益性行业科研专项(201203075-05) |
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| 摘 要： | 以梨优良矮化砧木‘中矮1号’新梢嫩茎为试材,根据白梨(Pyrus bretschneideri Rehd.)基因组预测的相关基因序列信息设计特异引物,克隆获得1个Katanin蛋白家族成员基因。序列分析显示该基因编码区全长1 566 bp,编码521个氨基酸,预测蛋白分子量约为57.2 kD,等电点为8.11,与其他物种的Katanin P60家族氨基酸序列具有78.01%~98.66%的相似性,将该基因命名为PcLUE1。实时荧光定量RT-PCR(qRT-PCR)分析显示,生长慢的矮化品种新梢快速生长时期PcLUE1的表达量显著高于生长快的乔化品种。石蜡切片显示,矮化品种与半矮化及乔化品种间茎组织细胞的排列方式和细胞大小存在较大差异。过表达PcLUE1的烟草植株与对照相比,株高降低,叶片变小,颜色变深,与‘中矮1号’中PcLUE1高表达效应极为相似,说明PcLUE1可能与‘中矮1号’的矮化有一定的关系。
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| 关 键 词： | 梨 Katanin蛋白家族 PcLUE1基因 矮化 |
Cloning and Functional Analysis of LUE1 Gene from'Zhongai 1'Pear |
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| HAO Ningning,DUAN Xuwei,OU Chunqing,CHEN Qiuju,WANG Fei,JIANG Shuling,MA Li.Cloning and Functional Analysis of LUE1 Gene from'Zhongai 1'Pear[J].Acta Horticulturae Sinica,2017,44(5):839-849. |
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| Authors: | HAO Ningning DUAN Xuwei OU Chunqing CHEN Qiuju WANG Fei JIANG Shuling MA Li |
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| Institution: | 1Institute of Pomology，Chinese Academy of Agricultural Sciences，Key Laboratory of Horticultural Crops Germplasm Resources Utilization，Ministry of Agriculture，Xingcheng，Liaoning 125100，China；2Institute of Forestry and Pomology，Beijing Academy of Agriculture and Forestry Sciences，Beijing 100193，China |
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| Abstract: | According to the genome database of Chinese white pear (Pyrus bretschneideri Rehd.),a 1 566 bp length gene was cloned from the tender stem of pear dwarfing rootstock'Zhongai 1'.This gene is predicted to encode a 57.2 kD protein,pI 8.11,comprising 521 amino acids.The deduced amino acid sequences has high homology (about 78.01％-98.66％ identity)to Katanin protein family,thus we named it PcLUE1.Real-time quantitative polymerase chain reaction (qRT-PCR) analysis showed that PcLUE1 expressed significantly higher in pear shoots in slowly growing dwarf cultivars than that in fast growing cultivars during rapid growing period.Paraffin sections of pear shoots showed that the cell arrangement and size had significant differences among dwarf,semi-dwarf and vigorous cultivars.The PcLUE1 over-expressed transgenic tobacco lines showed smaller and darker leaves than the controls,which is consistent with the phenotype of'Zhongai 1'.Taken together,these results suggested that PcLUE1 may contribute to the dwarfphenotype of'Zhongai 1'. |
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| Keywords: | pear Katanin Protein family PcLUE1 gene dwarf |
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