| 茶树烯醇酶基因CsENO的克隆及其在非生物胁迫中的表达分析 |
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| 引用本文： | 姚雪倩,陈丹,岳川,杨国一,王鹏杰,陈桂信,叶乃兴.茶树烯醇酶基因CsENO的克隆及其在非生物胁迫中的表达分析[J].园艺学报,2017,44(3):537-546. |
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| 作者姓名： | 姚雪倩 陈丹 岳川 杨国一 王鹏杰 陈桂信 叶乃兴 |
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| 作者单位： | 福建农林大学园艺学院，中国乌龙茶协同创新中心，福州 350002 |
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| 基金项目： | 福建省中国乌龙茶产业协同创新中心专项(闽教科[2015]75号)，国家自然科学基金项目(31600555)，福建省自然科学基金项目(2017J0106) |
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| 摘 要： | 从茶树‘黄旦’和‘金观音’抑制差减文库中筛选出1条烯醇酶(Enolase,ENO)cDNA片段序列,以‘铁观音’芽叶为材料克隆并验证该序列的全长编码c DNA序列。该序列全长1 753 bp,含有1个1 332 bp完整的开放阅读框,命名为CsENO,登录号KX962311。序列基因编码444个氨基酸,氨基酸序列分析发现,该c DNA与其他植物ENO高度保守,包含ENO特异结构域,与苹果(Malus×domestica)、白梨(Pyrus bretschneideri)的亲缘关系较近,相似性达84%。生物信息学预测结果显示,CsENO属于稳定的亲水蛋白,不存在跨膜结构,无信号肽,可能定位在过氧化体、溶酶体或核糖体等细胞质中,且具有多个磷酸化位点;二级结构主要由α–螺旋构成。实时荧光定量PCR结果表明,CsENO在低温、ABA、高盐、干旱逆境胁迫处理下均有表达,且表达受到不同程度的诱导。
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| 关 键 词： | 茶树 烯醇酶 基因克隆 逆境胁迫 |
Cloning of Enolase Gene CsENO and Its Expression Analysis Under Abiotic Stress in Tea Plant |
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| YAO Xueqian,CHEN Dan,YUE Chuan,YANG Guoyi,WANG Pengjie,CHEN Guixin,YE Naixing.Cloning of Enolase Gene CsENO and Its Expression Analysis Under Abiotic Stress in Tea Plant[J].Acta Horticulturae Sinica,2017,44(3):537-546. |
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| Authors: | YAO Xueqian CHEN Dan YUE Chuan YANG Guoyi WANG Pengjie CHEN Guixin YE Naixing |
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| Institution: | College of Horticulture，Fujian Agriculture and Forestry University，Collaborative Innovation Center of Chinese Oolong Tea Industry，Fuzhou 350002，China |
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| Abstract: | The cDNA fragment sequence encoding enolase (ENO) was obtained from the suppression subtractive library between Camellia sinensis ‘ Huangdan’ and Camellia sinensis ‘ Jinguanyin’,and then the full-length sequence of ENO was cloned and verified from C.sinensis ‘Tieguanyin’ and named as CsENO (Accession No.KX962311).The cDNA length of CsENO was 1 753 bp,containing a 1332 bp open reading frame (ORF) encoded 444 amino acid residues.Amino acid sequence analysis indicated that CsENO had the highly conserved regions in plants with specific structure domains of ENO and had the closer genetic relationship with Malus × domestica and Pyrus bretschneideri,which shared 84％ identity in amino acids.Bioinformatic prediction showed that CsENO belonged to the stability and hydrophilic protein with no transmembrane structure and signal peptide.It might be localized in other subcells and there were phosphorylation sites within the polypeptide chain.Secondary structure of CsENO was predicted to be mainly consisted of alpha helix.The quantitative real-time PCR showed that CsENO was all expressed in response to cold stress,ABA,high salinity as well as drought treatment.And its expression was induced by different degrees. |
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| Keywords: | tea plant enolase (ENO) gene cloning abiotic stress |
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