| 大白菜—结球甘蓝易位系‘AT9-1’和‘AT9-2’的获得及其遗传稳定性分析 |
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| 引用本文： | 毛清云,轩淑欣,顾爱侠,赵建军,申书兴,王彦华.大白菜—结球甘蓝易位系‘AT9-1’和‘AT9-2’的获得及其遗传稳定性分析[J].园艺学报,2017,44(5):871-880. |
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| 作者姓名： | 毛清云 轩淑欣 顾爱侠 赵建军 申书兴 王彦华 |
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| 作者单位： | 河北农业大学园艺学院，河北省蔬菜种质创新与利用重点实验室，河北省蔬菜产业协同创新中心，河北保定 071001 |
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| 基金项目： | 河北省科技支撑计划项目(16226304D-2)，国家自然科学基金项目(31171964;31572120)，河北省海外高层次人才百人计划项目(E2013100011)，国家重点研发计划项目(2016YFD0100204-17)，河北农业大学科研发展基金项目(1216006) |
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| 摘 要： | 为创建大白菜—结球甘蓝易位系,以大白菜—结球甘蓝9号单体异附加系(AC9)为试材,对其与大白菜亲本‘85-1’回交后代植株进行游离小孢子培养。利用结球甘蓝9号染色体相对应的C06连锁群特异的20个InDel标记对小孢子植株进行筛选,结合细胞学鉴定从中获得两个添加甘蓝9号染色体不同片段的易位系植株‘AT9-1’和‘AT9-2’。通过对InDel标记的加密设计,明确了易位系‘AT9-2’中甘蓝片段大小为1.03 Mb;利用大白菜A基因组10个连锁群上均匀分布的177个InDel标记对其进行分子鉴定,初步确定了易位系‘AT9-2’中甘蓝染色体片段位于大白菜1号染色体上。对易位系植株‘AT9-1’和‘AT9-2’分别进行自交、与大白菜亲本‘85-1’回交、与大白菜高代自交系‘14-28’和‘14-36’杂交,利用结球甘蓝C06连锁群特异的InDel标记对自交、回交、杂交后代进行鉴定,结果表明:两个易位系中甘蓝9号染色体片段的遗传稳定性非常低,易位系‘AT9-1’的自交、回交和杂交后代中含有甘蓝染色体片段的植株比例仅为8.9%、3.1%和2.8%;而‘AT9-2’仅为6.7%、1.6%和2.6%。
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| 关 键 词： | 大白菜 结球甘蓝 易位系 InDel标记 遗传稳定性 |
Obtaining and Genetic Stability of Chinese Cabbage-Cabbage Translocation Lines'AT9-1'and'AT9-2' |
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| MAO Qingyun,XUAN Shuxin,Gu Aixia,ZHAO Jianjun,SHEN Shuxing,WANG Yanhua.Obtaining and Genetic Stability of Chinese Cabbage-Cabbage Translocation Lines'AT9-1'and'AT9-2'[J].Acta Horticulturae Sinica,2017,44(5):871-880. |
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| Authors: | MAO Qingyun XUAN Shuxin Gu Aixia ZHAO Jianjun SHEN Shuxing WANG Yanhua |
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| Institution: | College of Horticulture，Hebei Agricultural University，Key Laboratory for Vegetable Germplasm Enhancement and Utilization of Hebei，Collaborative Innovation Center of Vegetable Industry in Hebei，Baoding，Hebei 071001，China |
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| Abstract: | To create Chinese cabbage-cabbage translocation lines,isolated microspore culture was carded out using the progeny derived from crossing between Chinese cabbage-cabbage alien addition line AC9 and Chinese cabbage'85-1'.Two double haploid translocation lines'AT9-1'and'AT9-2'with different fragments of cabbage chromosome 9 were screened out combining chromosome specific InDel markers and meiosis observation.The exogenetic fragment size of the translocation line'AT9-2'was further identified by additional InDel markers,with the value of 1.03 Mb.Using 177 InDel markers uniformly distributed on ten linkage groups of Chinese cabbage,the cabbage chromosome fragment of translocation lines'AT9-1'and'AT9-2'were selfed,backcrossed with the parent'85-1',and hybridized with Chinese cabbage inbred lines'14-28'and'14-36',and their offspring individuals were identified by the specific InDel markers from linkage group C06 of cabbage.The results showed that the introduced chromosome fragments from cabbage in the two translocation lines was instability and very low in genetics,with ratio of keeping entire exogenous fragment in their selfmg progenies,backcross progenies and two hybridization progenies of 8.9％,3.1％,2.8％ for'AT9-1'and 6.7％,1.6％,2.6％ for 'AT9-2',respectively. |
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| Keywords: | Chinese cabbage cabbage translocation line InDel marker genetic stability |
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