| 胡萝卜根中主要类胡萝卜素含量相关QTL的精细定位 |
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| 引用本文： | 欧承刚,孙婷婷,刘新艳,李成江,徐东辉,赵志伟,庄飞云.胡萝卜根中主要类胡萝卜素含量相关QTL的精细定位[J].园艺学报,2017,44(2):288-296. |
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| 作者姓名： | 欧承刚 孙婷婷 刘新艳 李成江 徐东辉 赵志伟 庄飞云 |
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| 作者单位： | 中国农业科学院蔬菜花卉研究所，农业部园艺作物生物学与种质创制重点实验室，北京 100081；宿州农业科学院，安徽宿州 234000 |
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| 基金项目： | 国家重点研发计划项目(2016YFD0100204-04)，国家科技支撑计划项目(2013BAD01B04)，中国农业科学院科技创新工程项目，国家自然科学基金项目(31272162) |
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| 摘 要： | 以白色的野生胡萝卜‘松滋野生’(Ws)和橘色的栽培胡萝卜品种‘Amsterdam’(Af)为亲本构建的回交重组自交系(BIL)为试材,基于低倍重测序技术开发SNP标记,构建了由1 976个Bin标记组成,包含29 435个SNP标记的遗传图谱。图谱总距离834.28 c M,平均图距0.42 c M。通过对胡萝卜肉质根中类胡萝卜素含量相关QTL分析,在连锁群LG04和LG08中检测到调控α–胡萝卜素、β–胡萝卜素、ζ–胡萝卜素、叶黄素、玉米黄质和总类胡萝卜素含量的主效QTL(M-QTL)2、2、3、2、2和2个,表型贡献率为11.47%~19.18%;另检测到调控α–胡萝卜素、β–胡萝卜素、ζ–胡萝卜素、玉米黄质和总类胡萝卜素含量的上位性QTL(E-QTL)1、1、2、1和1个,表型贡献率为2.50%~3.66%。在M-QTL显著区间内共检索到36个有功能注释的预测基因,其中Dck018297为ζ–胡萝卜素脱氢酶2基因,与调控β–胡萝卜素合成和总类胡萝卜素含量有关;Dck008006为乙烯响应因子2.2的同源基因,与调控α–胡萝卜素、ζ–胡萝卜素合成有关;Dck029898为转录因子b HLH135的同源基因,与调控玉米黄质合成有关。
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| 关 键 词： | 胡萝卜 类胡萝卜素 遗传图谱 精细定位 QTL |
Fine Mapping of QTLs Related to Main Carotenoids in Carrot Root |
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| OU Chenggang,SUN Tingting,LIU Xinyan,LI Chengjiang,XU Donghui,ZHAO Zhiwei,ZHUANG Feiyun.Fine Mapping of QTLs Related to Main Carotenoids in Carrot Root[J].Acta Horticulturae Sinica,2017,44(2):288-296. |
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| Authors: | OU Chenggang SUN Tingting LIU Xinyan LI Chengjiang XU Donghui ZHAO Zhiwei ZHUANG Feiyun |
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| Institution: | The Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Key Laboratory of Horticultural Crop Biology and Germplasm Innovation，Ministry of Agriculture，Beijing 100081，China；Suzhou Academy of Agricultural Sciences，Suzhou，Anhui 234000，China |
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| Abstract: | Based on whole-genome resequencing data,a high resolution genetic map was developed by using a set of backcross inbred lines (BILs) crossed by a wild species'Songzi'with a cultivar 'Amsterdam',and used to map QTLs for main carotenoids in carrot root.Finally,the genetic map was consisted of 1 976 Bins which contained 29 435 SNPs.The total genetic distance of map was 834.28 cM with an average interval of 0.42 cM between Bin markers.There were 2,2,3,2,2 and 2 main-effect QTLs (M-QTLs) associated with a-carotene,β-carotene,ζ-carotene,lutein,zeaxanthin and total carotenoid contents,respectively,with 11.47％-19.18％ phenotypic variance.There were 1,1,2,1 and 1 epistatic QTLs contributed to a-carotene,β-carotene,ζ-carotene,zeaxanthin and total carotenoid contents,respectively,with 2.50％-3.66％ phenotypic variance.A total of 36 predicted genes with function annotations were detected in M-QTLs.Among these,gene Dck018297 was ζ-carotene desaturase and associated with β-carotene biosynthesis and total carotenoid contents;gene Dck008006 homologouse to ERF2.2 was associated with a-carotene and zeaxanthin biosynthesis;gene Dck029898 homologouse to bHLH135 was associated with ζ-carotene biosynthesis.These three candidate genes might play central roles in the carotenoid biosynthesis in carrot root. |
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| Keywords: | carrot carotenoid genetic map fine mapping QTL |
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