| 桃PpSnRK1α在番茄中过表达对营养胁迫下植株生长的影响 |
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| 引用本文： | 罗静静,张亚飞,彭妍,彭福田,赵永飞,于雯.桃PpSnRK1α在番茄中过表达对营养胁迫下植株生长的影响[J].园艺学报,2017,44(4):644-652. |
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| 作者姓名： | 罗静静 张亚飞 彭妍 彭福田 赵永飞 于雯 |
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| 作者单位： | （1山东农业大学园艺科学与工程学院，作物生物学国家重点实验室，山东泰安 271018；2华中农业大学植物科学技术学院，武汉 430070） |
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| 基金项目： | 国家现代农业产业技术体系建设专项资金项目(CARS-31-3-03)，国家自然科学基金项目(31672099) |
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| 摘 要： | 以番茄(Solanum lycopericum)超表达桃SnRK1(蔗糖非发酵蛋白激酶–1)基因PpSnRK1α的株系及野生型为试材,研究在养分供应不足时SnRK1对植株生长的影响。结果表明,低营养条件下,转基因番茄叶片和根系中的Sn RK1酶活性比野生型高41.55%和39.46%;功能叶片的净光合速率平均比野生型高18.98%;低营养胁迫12 d的叶片SOD、POD、CAT活性比野生型高35.56%、28.85%和14.90%;根系活力比野生型高26.39%;茎和叶中氮磷含量显著高于野生型,钾含量两者差别不大,在根系中氮磷含量差别不大,而钾含量显著高于野生型,且氮素向地上部茎和叶中的分配比率增加。上述结果说明,在营养缺乏条件下,超表达PpSnRK1α可以提高番茄功能叶净光合速率,促进植株对氮素的吸收利用,从而延缓叶片衰老。
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| 关 键 词： | 桃 SnRK1 营养胁迫 番茄 净光合速率 养分含量 抗氧化酶 |
Effects of Overexpressing PpSnRK1α on the Growth of Tomato Plants Under Nutrient Stress |
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| LUO Jingjing,ZHANG Yafei,PENG Yan,PENG Futian,ZHAO Yongfei,YU Wen.Effects of Overexpressing PpSnRK1α on the Growth of Tomato Plants Under Nutrient Stress[J].Acta Horticulturae Sinica,2017,44(4):644-652. |
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| Authors: | LUO Jingjing ZHANG Yafei PENG Yan PENG Futian ZHAO Yongfei YU Wen |
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| Institution: | （1College of Horticulture Science and Engineering，Shandong Agricultural University，State Key Laboratory of Crop Biology，Tai’an，Shandong 271018，China；2College of Plant Science，Huazhong Agricultural University，Wuhan 430070，China） |
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| Abstract: | PpSnRKlα-overexpressing tomato (T2-3) and wild type tomato were used to study the effects of SnRK1 on the growth of tomato plants under nutrient stress.The results shows that:compared with wild type tomato,SnRK1 activity in leaves and roots increased by 41.55％ and 39.46％ of PpSnRKlα-overexpressing tomato under nutrient stress.The average photosynthetic rates increased by 18.98％ in mature leaves of T2-3 compared with wild type.After 12 days under low nutrient stress,the transgenic lines overexpressing PpSnRKlα showed better antioxidant enzymes and root activity.The SOD,POD,CAT activities and root activity were increased 35.56％ 8.85％ 14.90％ and 26.39％ compared with wild type.The content of N,P in the stem and leaf of T2-3 was higher than that of wild type plants,while there was no obvious difference in the K content.In the roots,there were no significant difference in the N,P content,while the content of K of transgenic line is obviously higher than that of wild type.In addition,more N was distributed to aboveground parts.These results above show that overexpressing PpSnRKlα can improve the photosynthetic rates and promote the absorption of nitrogen,and then delay the leaf senescence. |
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| Keywords: | peach SnRK1 nutrient stress tomato photosynthetic rate nutrient content antioxidant enzyme |
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