| 不同辣椒品种果实发育过程中DNA甲基化的MSAP分析 |
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| 引用本文： | 李宁,帕提古丽·艾斯木托拉,杨生保,王柏柯,唐亚萍,杨涛,余庆辉.不同辣椒品种果实发育过程中DNA甲基化的MSAP分析[J].新疆农业科学,2018,55(9):1601-1607. |
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| 作者姓名： | 李宁 帕提古丽·艾斯木托拉 杨生保 王柏柯 唐亚萍 杨涛 余庆辉 |
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| 作者单位： | 新疆农业科学院园艺作物研究所,乌鲁木齐 830091 |
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| 基金项目： | 新疆维吾尔自治区自然科学基金项目(2016D01B040) |
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| 摘 要： | 【目的】分析辣椒GB14和GB38果实发育过程中酶切位点的甲基化,为在分子水平上研究辣椒红素调控规律、在辣椒遗传育种中奠定理论基础。【方法】对辣椒果实发育成熟过程中的DNA甲基化修饰进行分析。【结果】DNA半甲基化条带比率与全甲基化条带比率均随着籽粒的生长发育而逐渐升高,两种辣椒果实绿熟期和红熟期基因组总体甲基化比例分别为36.51%、36.38%、35.57%和35.45%。对甲基化表现呈多态性变化的8个片段回收、测序分析表明,DNA甲基化发生的位点既存在于基因组的编码区,也存在于非编码区。【结论】在辣椒果实发育过程中,DNA甲基化修饰发生了巨大的变化;CCGG位点的半甲基化水平与辣椒红素积累呈正比。
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| 关 键 词： | 辣椒 果实发育 DNA甲基化 甲基化敏感扩增多态性分析 |
| 收稿时间： | 2018-07-27 |
MSAP Analysis of DNA Methylation in Different Capsicum annuum L.Varieties during Fruit Development |
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| LI Ning,Patiqul Asimutula,YANG Bao-sheng,WANG Bai-ke,TANG Ya-ping,YANG Tao,YU Qing-hui.MSAP Analysis of DNA Methylation in Different Capsicum annuum L.Varieties during Fruit Development[J].Xinjiang Agricultural Sciences,2018,55(9):1601-1607. |
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| Authors: | LI Ning Patiqul Asimutula YANG Bao-sheng WANG Bai-ke TANG Ya-ping YANG Tao YU Qing-hui |
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| Institution: | 1.College of Plant Science, Tarim University, Alar Xinjiang 843300, China |
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| Abstract: | 【Objective】 This project aims to carry out methylation analysis of restriction endonuclease sites in GB14 and GB38 fruit development of capsicum in the hope of providing a new insight for exploring the molecular mechanism of capsicum fruit development.【Method】The modification of DNA methylation during the development and maturation of Capsicum annuum L. fruit was studied. 【Result】The data showed that the ratios of DNA methylation and total methylation bands both increased gradually with seeds growth and development. The total methylation ratio of the two varieties of Capsicum annuum L.in green maturity period and red ripening period were 36.51%, 36.38%, 35.57% and 35.45%, respectively. Eight polymorphic fragments of methylation showed that the DNA methylation sites existed both in the coding region of the genome and in the non-coding region by the recovery and sequencing.【Conclusion】During the development of capsicum fruit, the modification of DNA methylation was greatly changed and the level of semi-methylation of CCGG loci was positively related to the accumulation of Capsicum annuum L. These results suggest a molecular level method for further studying the regulation of Capsicum annuum L., which provide a theoretical foundation for the breeding of Capsicum annuum L. |
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| Keywords: | Capsicum annuum L fruit development DNA methylation MSAP analysis |
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