| 3种不同广西家蚕品种幼虫培养蚕虫草的对比分析 |
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| 引用本文： | 吴婧婧.3种不同广西家蚕品种幼虫培养蚕虫草的对比分析[J].中国农学通报,2020,36(8):105-110. |
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| 作者姓名： | 吴婧婧 |
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| 作者单位： | 广西壮族自治区蚕业技术推广站,南宁 530007 |
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| 基金项目： | 农业部公益性行业专项(农业)科研专项项目“蚕桑资源高值化加工利用技术及设备研究与示范”(201403604);广西蚕业科学院青年基金 项目“蚕虫草培育技术的研究”(NO.201506)。 |
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| 摘 要： | 研究3种不同广西家蚕品种幼虫培养蚕虫草的对比分析,旨在筛选出适合生产优质高产蚕虫草的家蚕品种,为广西蚕桑产业资源综合利用提供参考依据。从3种不同的北虫草菌种中筛选出最适合的菌种,并将其接种至3种广西家蚕品种的幼虫培养蚕虫草,通过调查蚕虫草的形态、性状以及检测生物活性成分含量,来对比出适合作为蚕虫草培养生物材料的家蚕品种。结果表明：菌种A的家蚕蚕体和菌丝生长情况最优,可作为最佳接种的菌种;3种不同品种的家蚕培养的蚕虫草的形态与性状以‘两广二号（正交）’品种的各项指标均为最高,蚕虫草总质量为1.361 g、僵蚕体质量为1.028 g、子实体总数量为10.489根和子实体长度为9.411 cm;蚕虫草的虫草素和虫草腺苷的含量也以‘两广二号（正交）’品种为最高,分别为4.84 mg/g和1.79 mg/g;3种蚕虫草蚕底座的虫草含量比子实体的虫草素含量要高,其中‘两广二号（正交）’的蚕底座和子实体虫草素含量最高,分别为7.93 mg/g和4.79 mg/g;3种蚕虫草子实体的虫草腺苷含量要高于蚕底座的虫草腺苷含量,其中‘两广二号（正交）’的蚕底座和子实体的虫草腺苷含量最高,分别为3.51 mg/g和6.39 mg/g。研究结果表明,广西家蚕品种‘两广二号（正交）’所培养出的蚕虫草的指标和质量较优,可将‘两广二号（正交）’作为培养蚕虫草的优势资源。
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| 关 键 词： | 广西家蚕品种 蚕虫草 虫草素 虫草腺苷 对比分析 |
| 收稿时间： | 2019/2/15 0:00:00 |
| 修稿时间： | 2020/2/13 0:00:00 |
Comparative analysis of three different silkworm varieties of Guangxi in larval culture of Cordyceps militaris |
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| Wu Jingjing,Zhou Xiaoling,Dong Guiqing,Lu Chunxia,Liang Guiqiu,Chen Zhi,Lu Fei,Mao Hongbin,Shi Weitao.Comparative analysis of three different silkworm varieties of Guangxi in larval culture of Cordyceps militaris[J].Chinese Agricultural Science Bulletin,2020,36(8):105-110. |
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| Authors: | Wu Jingjing Zhou Xiaoling Dong Guiqing Lu Chunxia Liang Guiqiu Chen Zhi Lu Fei Mao Hongbin Shi Weitao |
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| Institution: | Guangxi Sericulture Technology Extension Station, Nanning 530007 |
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| Abstract: | The aim is to screen out the silkworm varieties suitable for producing high-quality and high-yield silkworm-hosted Cordyceps militaris, and to provide references for the comprehensive utilization of sericulture industry resources in Guangxi. The most suitable strain was screened out from three different strains of Cordyceps militaris, and inoculated into the larvae of three Guangxi silkworm varieties to cultivate silkworm-hosted Cordyceps militaris. Through investigating the morphology and characteristics of silkworm-hosted Cordyceps militaris and detecting the contents of biological active components, the most suitable silkworm variety for the culture of Cordyceps militaris was selected. The results showed that the silkworm body and the growth of mycelium of strain A were the best and could be used as the best inoculated strain. ‘Liangguang 2 (orthogonal)’ variety had the best morphology and characteristics of silkworm-hosted Cordyceps militaris in three silkworm varieties. The total mass of silkworm-hosted Cordyceps militaris was 1.361 g, the mass of batrytictic silkworm body was 1.028 g, the total number of sporophores was 10.489 and the length of sporophores was 9.411 cm. ‘Liangguang 2 (orthogonal)’ variety also had the highest content of cordycepin and adenosine in silkworm-hosted Cordyceps militaris, which was 4.84 mg/g and 1.79 mg/g, respectively. The contents of cordycepin in the three species of silkworm-hosted Cordyceps militaris pedestal were all higher than that in the sporophore. ‘Liangguang 2 (orthogonal)’ had the highest content of cordycepin in the silkworm-hosted Cordyceps militaris pedestal and sporophore, which was 7.93 mg/g and 4.79 mg/g, respectively. The contents of sporophore in the three species of silkworm-hosted Cordyceps militaris sporophore were all higher than that in the pedestal. ‘Liangguang 2 (orthogonal)’ had the highest content of cordyceps adenosine in the silkworm-hosted Cordyceps militaris pedestal and sporophore, which was 3.51 mg/g and 6.39 mg/g, respectively. The results showed that the Guangxi silkworm variety ‘Liangguang 2 (orthogonal)’ had better silkworm-hosted Cordyceps militaris index and quality, and could be used as the superior resource. |
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| Keywords: | Guangxi silkworm varieties silkworm Cordyceps militaris cordycepin sporophore comparative analysis |
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