Antibiotic resistance and molecular characteristics of <Emphasis Type="Italic">Staphylococcus aureus</Emphasis> isolated from backyard-raised pigs and pig workers |
| |
| Authors: | Asabe Halimat Momoh Jacob K P Kwaga Mohammed Bello Anthony K B Sackey Anders Rhod Larsen |
| |
| Institution: | 1.Department of Veterinary Public Health and Preventive Medicine,Ahmadu Bello University,Zaria,Nigeria;2.Department of Veterinary Medicine,Ahmadu Bello University,Zaria,Nigeria;3.National Center for Antimicrobial and Infection Control,Statens Serum Institut,Copenhagen,Denmark |
| |
| Abstract: | Staphylococcus aureus is a commensal and pathogenic bacterium with impact on public health and livestock industry. The study investigated nasal carriage, antibiotic resistance, and molecular characterization of S. aureus in pigs and pig workers. Nasal swabs from 300 backyard-raised pigs and 101 pig workers were used for the study. Resulting isolates were confirmed using MALDI-TOF MS, tested for antibiotic resistance, and three different multiplex PCRs were used to detect enterotoxin, mecA, spaA, scn, and pvl genes. spa typing was used to annotate the isolates into MLST clonal complexes (CC). Structured questionnaire was used to access possible risk factors for S. aureus carriage. The prevalence of S. aureus in pigs and pig workers were 5.3 and 12.9%, respectively. The isolates were resistant to beta-lactams (97%), tetracycline (62%), sulfonamide (52%), aminoglycoside (20.6%), fluoroquinolone (24%), and mupirocin (3.4%). Twenty seven (93%) of the isolates carried scn, 7(24%) pvl, and 12 (41%) enterotoxin genes, respectively. Questionnaire survey showed medical-related occupation of household members was associated (p?<?0.5) with S. aureus carriage. This study suggests the presence of human multidrug resistant strains of S. aureus, high carriage of pvl, and enterotoxin genes, and CC5, CC15, and CC152 were the CC-groups shared among pigs and pig workers. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
