The changes in digestive enzymes and hormones of gilthead seabream larvae (Sparus aurata, L 1758) fed on Artemia nauplii enriched with free methionine |
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| Authors: | Mehmet Naz Mustafa Türkmen |
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| Institution: | (1) Faculty of Fisheries Sciences, Mustafa Kemal University, Hatay, 31040, Turkey |
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| Abstract: | Variations in digestive enzymes and hormones during the larval development of gilthead seabream (Sparus aurata) fed on live prey (Artemia nauplii) enriched with free methionine were investigated for 16 days (from day 24 to day 40). Prior to initiation of the
experiment, newly hatched larvae were transferred from incubators to fiber glass tanks (300 l) with black walls and fed with
same diets until day 24. Each experiment was performed in triplicate. In the experimental group, the content of the free methionine
in the Artemia nauplii was increased by adding a 5.3 mM free methionine solution to the culture water during a 16-h enrichment period. The larvae of both the control and enriched-methionine
groups were sampled four times, with 4-day intervals between samplings, during a 16-day period. The larvae in the control
group had a significantly lower growth than those of the methionine group at the end of the study (P < 0.05). The highest trypsin activity and leucine aminopeptidase N/leucine–alanine peptidase ratios were observed in the
control group. A significant difference between bombesin activities in the treatment groups was not found at 5th minute after
the initiation of feeding (P > 0.05), but they were significant at 15th minute post-initiation of feeding (P < 0.05). A significant difference between the cholecystokinin levels of the treatment groups was found (P < 0.05). |
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| Keywords: | Alkaline phosphatase Aminopeptidase N Amylase Bombesin Cholecystokinin Digestive enzymes Leucine alanine peptidase Sparus aurata Trypsin |
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