| 不同生长调节物质对丹参愈伤组织的诱导效应 |
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| 引用本文： | 柳福智,董娟娥,梁宗锁.不同生长调节物质对丹参愈伤组织的诱导效应[J].中国农学通报,2005,21(11):202-204,275. |
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| 作者姓名： | 柳福智 董娟娥 梁宗锁 |
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| 作者单位： | 西北农林科技大学 陕西杨凌712100 |
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| 基金项目： | 陕西省十五科技攻关项目“主要药用植物的生长发育与有效成分积累研究”（2001KG01-G15-03）. |
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| 摘 要： | 以丹参(SalviamiltiorrhizaBunge)无菌苗的茎、叶为外植体,接种于附加2,4-D、NAA、6-BA及其组合的MS固体培养基上。结果表明:单独使用三种植物生长调节物质在一定浓度范围内均能产生愈伤组织;配合使用外源生长素和细胞分裂素,能获得出愈率高、生长快、质量好的愈伤组织;最佳组合的诱导培养基为MS 2,4-D2．0mg／L 6-BA1．0mg／L;2,4-D对丹参愈伤组织的诱导有促进作用;但当2,4-D的浓度在4mg／L以上时,对愈伤组织的生长有抑制作用。
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| 关 键 词： | 丹参 愈伤组织 生长调节物质 诱导 |
| 收稿时间： | 2005-04-03 |
| 修稿时间： | 2005-04-032005-04-09 |
A Study on the Effect of Different Growth Regulators on Callus Induction of Salvia miltiorrhiza Bunge |
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| Liu Fuzhi,Dong Juan'' e,Liang Zongsuo.A Study on the Effect of Different Growth Regulators on Callus Induction of Salvia miltiorrhiza Bunge[J].Chinese Agricultural Science Bulletin,2005,21(11):202-204,275. |
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| Authors: | Liu Fuzhi Dong Juan' e Liang Zongsuo |
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| Institution: | Northwest Agricultural and Forestry University, Yangling Shanxi 712100 |
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| Abstract: | Salvia miltiorrhiza Bunge aseptic stems and leaves were used as explants cultured in solid medium MS supplemented with 2,4-D, NAA, 6-BA or combinations of plant growth regulators with different concentrations. The results showed that all the three regulators could induce callus under certain concentration ranges. Better results could be obtained by combining three regulators, the optimum combination was Ms 2,4-D2.0mg/L 6-BA1.0mg/L. Higher concentration of 2,4-D (over 4mg/L) restrain callus growth. |
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| Keywords: | Salvia miltiorrhiza Bunge Callus Plant growth regulator Callus induction |
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