| 马比木根中喜树碱含量的HPLC测定方法 |
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| 引用本文： | 蒲尚饶,杨朗生,蹇凯,马明东.马比木根中喜树碱含量的HPLC测定方法[J].安徽农业科学,2010,38(6):2934-2935,2942. |
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| 作者姓名： | 蒲尚饶 杨朗生 蹇凯 马明东 |
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| 作者单位： | 四川农业大学都江堰分校,四川都江堰,611830;四川农业大学都江堰分校,四川都江堰,611830;四川农业大学都江堰分校,四川都江堰,611830;四川农业大学都江堰分校,四川都江堰,611830 |
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| 基金项目： | 四川农业大学院级攻关项目 |
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| 摘 要： | 目的]建立马比木根中喜树碱含量的测定方法。方法]采用高效液相色谱法。色谱柱为SHIM-PACKVP-ODS C18（250.0 mm×4.6 mm,5.0μm）,流动相为甲醇-水（50∶50,V/V）,流速1.0 ml/min,检测波长254 nm,柱温40℃。结果]喜树碱进样量在0.2～1.0μg时,与峰面积线性关系良好（r=0.999 5）;回收率（n=9）为99.8%～101.3%,RSD为0.56%～1.32%。结论]该方法简便、快速、准确,适用于马比木的质量控制。
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| 关 键 词： | 高效液相色谱法 喜树碱 马比木 |
Determination Method of Camptothecin Content in Roots of Nothapodytes pittosporoides (Oliv.) Sleum by HPLC |
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| PU Shang-rao et al.Determination Method of Camptothecin Content in Roots of Nothapodytes pittosporoides (Oliv.) Sleum by HPLC[J].Journal of Anhui Agricultural Sciences,2010,38(6):2934-2935,2942. |
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| Authors: | PU Shang-rao |
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| Institution: | Dujiangyan School of Sichuan Agricultural University;Dujiangyan;Sichuan 611830 |
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| Abstract: | Objective] The research aimed to establish a HPLC method for determining the content of camptothecin in the roots of Nothapodytes pittosporoides(Oliv.) Sleum.Method] The separation was made on a SHIM-PACK VP-ODS C18 column(250.0 mm×4.6 mm,5.0 μm)with methanol-water(50∶50,V/V)as the mobile phase at a flow rate of 1.0 ml/min and 40 ℃.The detective wavelength was set at 254 nm.Result] The simple size of camptothecin had a good linear relationship with the peak areas in the range of 0.2-1.0 μg(r=0.999 5).The recovery was 99.8%-101.3%,with RSD of 0.56%-0.98%(n=9).Conclusion] This method is rapid,simple and accurate,which was suitable for the quality control of Nothapodytes pittosporoides(Oliv.) Sleum. |
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| Keywords: | HPLC Camptothecin Nothapodytes pittosporoides (Oliv ) Sleum |
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