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| 禽巴氏杆菌C48-1外膜蛋白H基因克隆及序列分析 | |
| 引用本文： | 曹素芳,黄青云,韩先干,陈红英,邓宪方.禽巴氏杆菌C48-1外膜蛋白H基因克隆及序列分析[J].中国预防兽医学报,2006,28(3):253-256. |
| 作者姓名： | 曹素芳 黄青云 韩先干 陈红英 邓宪方 |
| 作者单位： | 1. 华南农业大学,广东,广州,510642;郑州牧业工程高等专科学院,河南,郑州,450011 2. 华南农业大学,广东,广州,510642 3. 河南农业大学,河南,郑州,450002 |
| 摘 要： | 根据已发表的外膜蛋白基因核苷酸序列设计一对特异性引物，应用PCR方法扩增出禽巴氏杆菌5：A C48-1株的ompH全长片段，将ompH进行T—A克隆、序列测定和分析。结果表明，ompH全长1597bp，含有一个1056bp的开放性阅读框架（ORF），编码352个氨基酸，前20个氨基酸组成信号肽。与已知的15个血清型及痘苗株CU的ompH的核苷酸及其推导的氨基酸序列比较，核苷酸同源性在51．5％～98．7％之间，氨基酸同源性在39．3％～98，7％之间。氨基酸多序列比较显示，血清型特异性抗原表位位于60～80位和200～220位氨基酸处。 |
| 关 键 词： | 禽巴氏杆菌 外膜蛋白 序列分析 同源性 |
| 文章编号： | 1008-0589(2003)03-0253-04 |
| 收稿时间： | 2005-04-12 |
| 修稿时间： | 2005年4月12日 |
Cloning and sequencing of outer membrane protein H gene of avian pasteurella multocida C48-1 |
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| CAO Su-fang,HUANG Qing-yun,HAN Xian-gan,CHEN Hong-ying,DENG Xian-fang.Cloning and sequencing of outer membrane protein H gene of avian pasteurella multocida C48-1[J].Chinese Journal of Preventive Veterinary Medicine,2006,28(3):253-256. | |
| Authors: | CAO Su-fang HUANG Qing-yun HAN Xian-gan CHEN Hong-ying DENG Xian-fang |
| Institution: | 1. South China Agriculture University. Guangzhou 510642, China; 2. College of Zhengzhou Animal Husbandry Engineering, Zhengzhou 450011, China; 3. Henan Agriculture University, Zhengzhou 450002. China |
| Abstract: | According to ompH of pasteurella multocida published in GenBank, a pair of primers were designed. Using the primers, full-length ompH of C48-1 was amplified by PCR. It was cloned into pMD18-T vector and then sequenced. The result showed that the full-length ompH composed 1 597 bp.The coding region of ompH is 1056 bp at length. The predicted primary protein is composed of 352 amino acid with a 20-amino-acid signal peptide, After comparing with ompH from 15 pasteurella multocida serotype as well as the CU vaccine strain, it showed that nucleotide sequence homologies were 51.5 % to 98,7 % and the deduced amino acid homologies were 39,3% to 98.7%. By amino acid sequence alignment of ompH, two hypervariable regions-amino acids 60 to 80 and 200 to 220 which contain serotype specific epitomes and may induce protective antibodies were identified. |
| Keywords: | avian pasteurlla multocida outer membrane protein sequence analysis homology |
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