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| 影响根癌农杆菌介导的高效水稻遗传转化的相关因素分析 | |
| 引用本文： | 黄红梅,杨永智,张治国.影响根癌农杆菌介导的高效水稻遗传转化的相关因素分析[J].西北农林科技大学学报(社会科学版),2004,32(9):4-8. |
| 作者姓名： | 黄红梅 杨永智 张治国 |
| 作者单位： | 1. 中国农业科学院,生物技术研究所,北京,100081;西北农林科技大学,生命科学学院,陕西,杨凌,712100 2. 中国农业科学院,生物技术研究所,北京,100081 3. 西北农林科技大学,生命科学学院,陕西,杨凌,712100 |
| 基金项目： | 国家863计划项目(2001AA225051) |
| 摘 要： | 以水稻基因组测序品种日本晴(OryzasativaL.ssp.japonica)为材料,对根癌农杆菌介导的水稻转化系统进行了优化。结果表明,水稻种子经质量分数2.5%的次氯酸钠灭菌30min后,愈伤组织诱导率最高;所获得的胚性愈伤组织经过6～7d的继代培养后转化率达到95%;感染液菌浓度OD600值为0.145～0.190时,转化效率高;将抗性愈伤组织进行7d的暗培养可使分化效率提高到85%。应用本转化系统已经获得了大量的转化体,GUS表达率达到30%,并且3个月即可获得转化植株。 |
| 关 键 词： | 根癌农杆菌 水稻 遗传转化 |
| 文章编号： | 1671-9387(2004)09-0004-05 |
| 收稿时间： | 2004/3/22 0:00:00 |
| 修稿时间： | 2004年3月22日 |
Analysis of factors influencing highly efficient Agrobacterium-mediated rice transformation |
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| HUANG Hong-mei.Analysis of factors influencing highly efficient Agrobacterium-mediated rice transformation[J].Journal of Northwest Sci-Tech Univ of Agr and,2004,32(9):4-8. | |
| Authors: | HUANG Hong-mei |
| Institution: | HUANG Hong-mei~ |
| Abstract: | The rice transformation mediated by Agrobacterium tumefaciens was optimized by using Oryza sativa L.ssp.japonica cultivars,which is the matrerial of rice genome sequencing.The main results were as following:The frequency of callus induction is the highest when seeds sterilized in 2.5% sodium hypochlorite solution for 30 minutes;The transformation efficiency is the highest when embryogenic calli are subcultured for 6-7 days;the OD_(600)=0.145-0.190 of bacteria concentration is the best for callus transformation;with the 7-day cultivation in dark,the differentiation efficiency of the resistant calli can be improved to 85%.A lot of transformants have been obtained by using the transformation system in three months.GUS gene expression frequency is up to 30%. |
| Keywords: | Agrobacterium tumefaciens Oryza sativa L genetic transformation |
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