| 矮牵牛CHS基因启动子克隆、序列分析及植物表达载体构建 |
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| 引用本文： | 高水平,范丙友,史国安,贾小平,孔祥生.矮牵牛CHS基因启动子克隆、序列分析及植物表达载体构建[J].北方园艺,2010(17):155-158. |
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| 作者姓名： | 高水平 范丙友 史国安 贾小平 孔祥生 |
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| 作者单位： | 河南科技大学,林学院,河南,洛阳,471003;河南科技大学,农学院,洛阳市牡丹生物学重点实验室,河南,洛阳,471003 |
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| 基金项目： | 国家自然科学基金，林木、花卉遗传育种教育部重点实验室开放基金 |
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| 摘 要： | 应用PrimerPremier5.0软件,根据GenBank数据库报道的查尔酮合成酶基因启动子序列(EF199747)设计1对特异性PCR扩增引物,以矮牵牛品种‘午夜蓝色’叶片总DNA为模板,用TaqDNA聚合酶成功扩增出1条约0.5kb的DNA片段,回收该片段并连接到pMD18-T载体上。结果表明:经测序该启动子片段长550bp;bl2seq分析结果表明该启动子与目标序列相似性高达100%;PLACE在线分析显示在克隆片段中含有TATAbox、CAATbox、capsite、antherbox、box1、box2、Gbox及TACPyAT-box等顺式元件;并构建了矮牵牛CHS基因启动子融合标记基因GUS的植物表达载体pPhCHS::GUS。
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| 关 键 词： | 矮牵牛 CHS基因启动子 克隆 序列分析 植物表达载体 |
Cloning and Sequence Analysis of Promoter of Chalcone Synthase Gene from Petunia Hybrida and Construction of Plant Expression Vector |
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| GAO Shui-ping,FAN Bing-you,SHI Guo-an,JIA Xiao-ping,KONG Xiang-sheng.Cloning and Sequence Analysis of Promoter of Chalcone Synthase Gene from Petunia Hybrida and Construction of Plant Expression Vector[J].Northern Horticulture,2010(17):155-158. |
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| Authors: | GAO Shui-ping FAN Bing-you SHI Guo-an JIA Xiao-ping KONG Xiang-sheng |
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| Institution: | GAO Shui-ping1,FAN Bing-you2,SHI Guo-an2,JIA Xiao-ping2,KONG Xiang-sheng2 (1.College of Forestry,Henan University of Science and Technology,Luoyang,Henan 471003,2.College of Agriculture,Luoyang Key Laboratory of Peony Biology,Henan 471003) |
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| Abstract: | Based on the Primer Premier 5.0software,apair of specific PCR primers was designed according to promoter sequence of CHS gene of Petunia hybridareported in GenBank(EF199747).A DNA fragment about 0.5kb was successfully amplified with the genome DNA of Petunia hybridcultivar'Midnight'as template and Taq polymerase as DNA polymerase.The purified fragment of PCR products was ligated with pMD18-T vector,and the sequencing result showed that the length of the promoter of CHS gene of Petuniah ybrid was 550bp.Bl2se... |
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| Keywords: | Petunia hybrid promoter of CHSgene cloning sequence analysis plant expression vector |
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