| 内生解淀粉芽孢杆菌TB2液体发酵条件的研究 |
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| 引用本文： | 郭龙涛,邱思鑫,蔡学清,胡方平.内生解淀粉芽孢杆菌TB2液体发酵条件的研究[J].热带作物学报,2010,31(2):259-264. |
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| 作者姓名： | 郭龙涛 邱思鑫 蔡学清 胡方平 |
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| 作者单位： | 1. 福建农林大学植物保护学院,福州,350002
2. 福建省农业科学院蔬菜研究中心,福州,350013 |
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| 基金项目： | 国家自然科学基金资助项目，福建省发改委资助项目 |
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| 摘 要： | 采用单因素试验和正交试验对具有广谱拮抗作用的内生解淀粉芽孢杆菌TB2菌株的摇瓶发酵培养基和发酵条件进行优化。结果表明,最佳培养基组份为:玉米淀粉1.0%、豆粉1.0%、(NH4)2SO40.5%、MgSO4·7H2O0.1%、FeSO4·7H2O0.03%、K2HPO4·3H2O0.02%、KH2PO40.01%;培养条件为:初始pH值为7.5,温度35℃,接种量3%,装液量50mL/250mL三角瓶,摇床转速200r/min,发酵周期22～26h。优化条件下发酵水平活菌数达到8.52×1010cfu/mL。
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| 关 键 词： | 内生解淀粉芽孢杆菌TB2 发酵 单因素试验 正交试验 |
Optimization of Conditions for Liquid Fermentation of Endophytic Bacillus amyloliquefaciens TB2 |
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| Guo Longtao,Qiu Sixin,Cai Xueqing and Hu Fangping.Optimization of Conditions for Liquid Fermentation of Endophytic Bacillus amyloliquefaciens TB2[J].Chinese Journal of Tropical Crops,2010,31(2):259-264. |
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| Authors: | Guo Longtao Qiu Sixin Cai Xueqing and Hu Fangping |
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| Institution: | Guo Longtao1,Qiu Sixin2,Cai Xueqing1,Hu Fangping1 1 College of Plant Protection,Fujian Agriculture , Forestry University,Fuzhou 350002,China,2 Vegetable Research Centre,Fujian Academy of Agricultural Sciences,Fuzhou 350013 |
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| Abstract: | The antagonistic endophytie Bacillus amyloliquefaciens strain TB2 was propagated in shake flask cultures, and the optimum medium and conditions for its liquid fermentation were determined by using single factor and orthogonal experiments. The results showed that the optimum medium compositions were as follows:corn starch, 1.0%; soybean powder, 1.0%; (NH_4)_2SO_4, 0.5%; MgSO_4·7H_2O, 0.1%; FeSO_4·7H_2O, 0.03%;K_2HPO_4·3H_2O, 0.02%; KH_2PO_4, 0.01%; and that the optimum fermentation conditions were: the initial pH of medium adjusted to 7.5 prior to sterilization; incubation temperature, 35℃; inoculum volume, 3%; medium volume, 50 ml; shake flask, 250 ml Erlenmeyer flask; rotation speed, 200 rpm; fermentation cycle, 22～26 hr.Under these fermentation conditions the strain TB2 could be propagated at a cell density of upto 8.52×10~(10) cfu/ml. |
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| Keywords: | endophytic Bacillus amyloliquefaciens TB2 fermentation single factor experiment orthogonal experiment |
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