| 铁皮石斛试管苗快繁体系 |
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| 引用本文： | 王春,郑勇平,罗蔓,周芳勇,余琼芳.铁皮石斛试管苗快繁体系[J].浙江农林大学学报,2007,24(3):372-376. |
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| 作者姓名： | 王春 郑勇平 罗蔓 周芳勇 余琼芳 |
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| 作者单位： | 1.浙江森禾种业股份有限公司,浙江杭州310021 |
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| 基金项目： | 浙江省科学技术重点项目(2005C22082) |
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| 摘 要： | 铁皮石斛Dendrobium officinale是一种名贵药用植物,野生资源亟待保护,必须通过试管苗快繁技术研究,才能满足人们对它的需要。通过不同基本培养基对铁皮石斛进行种子发芽试验,不同消毒方法对茎尖进行存活率试验,不同植物生长调节物质组合对不定芽、原球茎和壮苗及根系的诱导试验的结果表明:铁皮石斛胚培养最佳培养基为1 2 MS(Murashige and Skoog)+添加物A+ 25 gL- 1蔗糖; 4种消毒方法中最佳消毒方法为B,外植物茎尖存活率可达76.2%。不定芽诱导最佳培养基为1 2MS+ 1.5 mgL- 1 6-苄基腺嘌呤(BA)+ 0.25 mgL- 1萘乙酸(NAA),诱导倍数4.7。原球茎诱导最佳培养基为1 2 MS+ 1.0 mgL- 1 BA+ 0.5mgL- 1 NAA,诱导率为58%。不定芽最佳生根培养基为1 2MS+ 0.5 mgL- 1 NAA,生根率为100%,平均生根数为7.1条。建立了铁皮石斛试管苗快繁体系,为其广泛应用提供了依据。图1表5参11
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| 关 键 词： | 植物学 铁皮石斛 组织培养 不定芽 原球茎 |
| 收稿时间： | 2006-06-07 |
Micropropagation of Dendrobium officinale |
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| WANG Chun,ZHENG Yong-ping,LUOMan,ZHOUFang-yong,YUQiong-fang.Micropropagation of Dendrobium officinale[J].Journal of Zhejiang A&F University,2007,24(3):372-376. |
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| Authors: | WANG Chun ZHENG Yong-ping LUOMan ZHOUFang-yong YUQiong-fang |
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| Institution: | 1.Zhejiang Senhe Seed Co., Ltd, Hangzhou 310020, Zhejiang, China |
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| Abstract: | Dendrobium officinale(iron-sheet dendrobium) is a rare plant species for medical use. Its present resource cannot satisfy the needs. Tissue culture is a quick and convenient path to produce more iron-sheetdendrobium. The tiny seeds were sowed on the basic culture medias of MS(Murashige and Skoog), half of MS(1 2 MS), Hyponexl and Hyponex 2. And the fresh stems were sterilized in differentways, then cultured in the basic media added NAA or BA in different ammounts. The resluts showed that 1 2 MS+ A(the additive substance)+ 25 gL- 1 sugar was the best medium for seeding; Method B was the best disinfection with the livability 76.2%; 1/2 MS+ 1.5 mgL- 1 BA+ 0.25 mgL- 1 NAA was better for adventitious buds induction,with 4.7 buds on each explant; 1 2 MS+ 1.0 mgL- 1BA+ 0.5 mgL- 1NAA was fine for protocorm induction,with the rate of 8%; 1/2 MS+ 0.5 mgL- 1 NAAwas betterfor rooting, with a 100% rate and 7.1 roots on each explants. The proliferation systerm can enlarge the application of Dendrobium officinale.Ch, 1 fig. 5 tab. 11ref.] |
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