| 4种石斛水提物对人宫颈癌HelaS3细胞和肝癌HepG2细胞的抑制作用 |
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| 引用本文： | 鲍丽娟,王军辉,罗建平.4种石斛水提物对人宫颈癌HelaS3细胞和肝癌HepG2细胞的抑制作用[J].安徽农业科学,2008,36(36). |
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| 作者姓名： | 鲍丽娟 王军辉 罗建平 |
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| 作者单位： | 合肥工业大学生物与食品工程学院,安徽合肥,230009 |
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| 基金项目： | 合肥工业大学青年基金，合肥工业大学大学生创新基金 |
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| 摘 要： | 目的]为进一步开发石斛药用资源提供基础数据。方法]将人宫颈癌细胞HelaS3和肝癌HepG2细胞用含10%新生小牛血清的RPMI-1640培养基在37℃和5%CO2条件下培养,比较了霍山石斛、铁皮石斛、金钗石斛和马鞭石斛水提物对HelaS3和HepG2细胞的体外抑制作用。结果]4种石斛水提物对HelaS3细胞和HepG2细胞均有不同程度的抑制作用,且在所选浓度范围内对剂量和时间有依赖性。4种石斛水提物对HelaS3细胞的IC50分别是11.63、17.71、15.11和5.89 mg/ml;对HepG2细胞的IC50分别是10.40、22.95、3.80和17.76 mg/ml。显微观察显示,处理组的活细胞多呈红色,核呈固缩状或圆珠状,有明显的染色质凝集和细胞凋亡特征。结论]剂量效应分析表明,对HelaS3细胞而言,马鞭石斛水提物抑瘤效果较好;对HepG2细胞而言,金钗石斛水提物抑瘤效果较好。
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| 关 键 词： | 霍山石斛 铁皮石斛 金钗石斛 马鞭石斛 肿瘤细胞 抑制作用 |
Inhibitory Effects of Water Extracts from Four Species of Dendrobiums on HelaS3 Cells and HepG2 Cells |
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| Abstract: | Objective] The study was to provide the foundation data for further development of Dendrobium medicinal resources.Method] HelaS3 cells and HepG2 cells were put on the RPMI-1640 medium with 10% new newborn calf serum and cultured under 37 ℃ and 5% CO2 to compare in vitro inhibitory effects of water extracts from Dendrobium huoshanense,D.candidum,D.nobile and D.fimbriatum on HelaS3 cells and HepG2 cells.Result] The water extracts from 4 species of Dendrobium could inhibit the growth of HelaS3 cells and HepG2 cells to different extent,with the inhibitory effects being dose-dependent and time-dependent in the tested concn.range.The IC50 of 4 species of Dendrobium on HelaS3 cells were 11.63,17.71,15.11 and 5.89 mg/ml,resp.and that on on HepG2 cells were 10.40,22.95,3.80 and 17.76 mg/ml,resp.The microscope observations showed that most of the living cells treated with extracts appeared red,with pycnosis of nucleu,agglutination of chromatin and occurrence of apoptosis induced by extracts.Conclusion] The dose effect analysis showed that the tumor inhibiting effect of water extracts from D.fimbriatum on HelaS3 cells was better and that from D.nobile HepG2 was better. |
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| Keywords: | Dendrobium huoshanense D candidum D nobile D fimbriatum Tumor cells Inhibitory effects |
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