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不同生长时期桑叶游离氨基酸含量变化
引用本文:甄占萱,杨贵明,高妍夏,李松涛,马博辉.不同生长时期桑叶游离氨基酸含量变化[J].北方蚕业,2014(3):10-12.
作者姓名:甄占萱  杨贵明  高妍夏  李松涛  马博辉
作者单位:1. 承德医学院蚕业研究所
2. 承德医学院中药研究所,河北承德,067000
摘    要:以坎下白桑、辽桑和慈山3个桑树品种为供试材料,6-9月间4次采样,以异硫氰酸苯酯为柱前衍生化试剂,采用反相高效液相色谱法测定桑叶游离氨基酸种类和含量。结果表明,桑叶中游离氨基酸总量和人体必需氨基酸总量,6月时分坎下白桑明显高于另外2个桑品种,7月分3个桑品种接近,8月分均降到较低水平,9月分均又回升并接近7月的水平。春季含量较高的丝氨酸到初霜时降低,春季含量较低的苏氨酸到初霜时升高。不同生长时期不同品种桑叶游离氨基酸种类和含量均有一定差别。

关 键 词:不同品种  生长时期  桑叶  游离氨基酸  含量变化

Content Variation of Free Amino Acid in Varieties of Mulberry Leaves at Different Growth Stages
ZHEN Zhanxuan,YANG Guiming,GAO Yanxia,LI Songtao,MA Bohui.Content Variation of Free Amino Acid in Varieties of Mulberry Leaves at Different Growth Stages[J].North Sericulture,2014(3):10-12.
Authors:ZHEN Zhanxuan  YANG Guiming  GAO Yanxia  LI Songtao  MA Bohui
Institution:ZHEN Zhanxuan, YANG Guiming , GAO Yanxia , LI Songtao , MA Bohui (Institute of Sericulture . Institute of Chinese Traditional Medicine Chengde Medical University, Chengde Hebei 067000, China)
Abstract:The mulberry leaves of Kanxiabai, Liaosang and Cishan were collected four times between June and September. Reversed--phase high performance liquid chromatography with precolumn Phenylisothiocyanate derivatization was used to analyze free amino acids type and content in mulberry leaves. The results showed that the total free amino acid and total essential amino acid in Kanxiabai was higher than other two species in June, but similar in July, and all down to a lower level in August, in September rebounded close to the level in July. The content of Serine was high in spring, but decreased at first frost period. The content of Threonine was low in spring, and then increased at first frost period. The types and content of free amino acid in varieties of mulberry leaves at different growth periods were different.
Keywords:different varieties  growth stages  mulberry leaves  free amino acids  content variation
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