| 一种少量提取植物组织DNA的快速方法 |
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| 引用本文： | 李维平,赵文明,雷 萍.一种少量提取植物组织DNA的快速方法[J].西北农林科技大学学报(社会科学版),2002,30(6):125-128. |
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| 作者姓名： | 李维平 赵文明 雷 萍 |
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| 作者单位： | 西安交通大学,生命科学与技术学院,陕西,西安,710049 |
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| 基金项目： | 国家自然科学基金资助项目(39670478);西安交通大学博士学位论文基金(DFXJU2000-10) |
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| 摘 要： | 为了从植物组织中快速提取核酸,从多种DNA提取方法中遴选出2种基本方法。通过优化基本方法的操作步骤、试剂用量与作用时间,建立了一种改良的DNA快速提取方法。在此方法中,用氯仿代替液氮或提取液进行植物组织研磨,之后加入提取液抽提DNA,并用氯仿-异戊醇反复抽提,可显著降低蛋白质含量;通过应用RNA酶可获得纯度较高的DNA。该方法的特点是在室温下即可进行简便操作,快速(提取核酸的过程仅需30min),污染器皿少,实用性强,提取物产量高,纯度好。
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| 关 键 词： | DNA 提取方法 植物组织 |
| 文章编号： | 1671-9387(2002)06-0125-04 |
| 收稿时间： | 2001/10/19 0:00:00 |
| 修稿时间： | 2001年10月19 |
Rapid method for extracting DNA from plant issues |
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| LI Wei ping,ZHAO Wen ming,LEI Ping.Rapid method for extracting DNA from plant issues[J].Journal of Northwest Sci-Tech Univ of Agr and,2002,30(6):125-128. |
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| Authors: | LI Wei ping ZHAO Wen ming LEI Ping |
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| Institution: | (Life Science and Technology college,Xian Jiaotong University,Xian City,Shaanxi,710049,China) |
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| Abstract: | Two methods were selected from various DNA isolated methods for extracting DNA from plant tissues rapidly.Through optimizing the steps,dosage of reagents and manipulating time,a reformative method is formed for extracting DNA rapidly.In our method,chloroform is replaced with liquid nitrogen,extracting reagent in which plant issues are skived was added, chloroform-isoamyl alcohol is used to extract DNA repeatedly,high yield of DNA is gained.Using RNase,varified DNA could be obtained.The trait of the method is simple to apply in room temperature,time-saving(30 min),less polluting,adaptable for extracting DNA from different plant materials and effective. |
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| Keywords: | DNA extraction and isolation method plant issues |
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