| 甲硝唑人工抗原的制备及其ELISA检测 |
| |
| 引用本文： | 俎晶晶,屈凌波,孙武勇.甲硝唑人工抗原的制备及其ELISA检测[J].动物医学进展,2007,28(1):11-13. |
| |
| 作者姓名： | 俎晶晶 屈凌波 孙武勇 |
| |
| 作者单位： | 1. 郑州大学化学系,河南郑州,450052
2. 中国药科大学药学院,江苏南京,210009 |
| |
| 基金项目： | 河南省高校杰出科研创新人才工程项目 |
| |
| 摘 要： | 采用碳二亚胺法将甲硝唑半抗原与牛血清白蛋白(BSA)连接制备人工免疫原,同样方法将其与卵清蛋白(OVA)连接制备人工包被原.经紫外扫描分析,两种方法合成的免疫原和包被原的结合比分别为8∶1、2∶1和6∶1、2∶1;动物免疫试验分析偶联物,小鼠抗体效价达1∶5 000,与3种硝基咪唑类药物的交叉反应率均小于10%.表明制备的抗体可以用于甲硝唑残留的检测,同时为检测试剂盒的研制奠定了基础.
|
| 关 键 词： | 甲硝唑 人工抗原 碳二亚胺法 |
| 文章编号： | 1007-5038（2007）01-0011-03 |
| 收稿时间： | 2006-10-19 |
| 修稿时间： | 2006年10月19 |
Synthesis and Identification of Metronidazole Artificial Antigen and the Development of ELISA Method |
| |
| ZU Jing-jing,QU Ling-bo,SUN Wu-yong.Synthesis and Identification of Metronidazole Artificial Antigen and the Development of ELISA Method[J].Progress In Veterinary Medicine,2007,28(1):11-13. |
| |
| Authors: | ZU Jing-jing QU Ling-bo SUN Wu-yong |
| |
| Abstract: | Two methods of metronidazole artificial hapten was reported in this paper.The immunogen,met- ronidazole-BSA,was synthesized by carbodimide method.The coating antigen,metronidazole-OVA,was synthesized by the same method.The couple rates of the immunogens and coating antigens are 8:1,2:1 and 6:1,2:1,respectively.It showed the titer of Balb/c mice was 1:5000,and the cross-reaction was lower than 10% comparing to the other three Nitromidazoles.The results demonstrated that the two artifi- cial antigens were successfully synthesized,which provides a basis for next monoclonal antibody prepara- tion and ELISA kit development. |
| |
| Keywords: | Metronidazole artificial antigen carbodimide methed |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
