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牦牛DNA分子遗传标记的研究
引用本文:马志杰,钟金城,陈智华,罗晓林.牦牛DNA分子遗传标记的研究[J].中国牛业科学,2006,33(1):43-48.
作者姓名:马志杰  钟金城  陈智华  罗晓林
作者单位:1. 青海省畜牧兽医科学院,畜牧研究所,青海,西宁,830001;西南民族大学,生命科学与技术学院,四川,成都,610041
2. 西南民族大学,生命科学与技术学院,四川,成都,610041
3. 青海省畜牧兽医科学院,畜牧研究所,青海,西宁,830001
摘    要:本文在归纳总结DNA分子遗传标记的类型、特点和检出方式的基础上,分析讨论了动物遗传育种研究中常用的DNA分子遗传标记如RFLP标记、VNTR标记、AFLP标记、PCR-SSCP标记、RAPD标记、SNPs标记、微卫星标记、DNA指纹标记及mtDNA标记等在牦牛遗传育种研究中的研究现状和存在的问题,指出DNA分子标记在牦牛遗传育种研究中已渗透到牦牛品种的起源、演化和分类研究;牦牛品种间或品种内个体间以及与其他种间亲缘关系鉴定;犏牛雄性不育的机理研究及牦牛经济性状功能基因的克隆和多态性研究等方面。最后探讨了今后的研究趋势和发展前景。

关 键 词:牦牛  DNA分子  标记
文章编号:1001-9111(2006)01-0043-06
收稿时间:2005-10-24
修稿时间:2005年10月24

Study on the DNA Molecular Markers of Yak
MA Zhi-jie,ZHONG Jin-cheng,CHEN Zhi-hua,LUO Xiao-lin.Study on the DNA Molecular Markers of Yak[J].China Cattle Science,2006,33(1):43-48.
Authors:MA Zhi-jie  ZHONG Jin-cheng  CHEN Zhi-hua  LUO Xiao-lin
Institution:1. Institute of Animal Science, Qinghai Academy of Animal Science and Veterinary Medicine, Xining, Qinghai 810003, China;2. College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan 610041, China
Abstract:In this paper,based on the types,characteristics and detection methods of various kinds of DNA molecular markers,the present situation and existing problems in yak genetic and breeding research of DNA molecular marker analyzing methods commonly used in the animal breeding and genetic research,such as RFLP,VNTR,AFLP,PCR-SSCP,RAPD,SNPs,microsatellite marker,DNA fingerprints and mtDNA markers etc.,have been discussed in detail,which indicated that the DNA molecular marker analysis had been involved in the research of yak origin,evolution,classification,parentage test among yak breeds and between yak and other animals,the mechanisms of male sterilization,the cloning of function genes related with economic traits,and the polymorphisms etc.The future research development and perspectives of yak breeding were also elucidated.
Keywords:Yak  DNA molecular  Marker
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