| 荧光定量PCR技术研究进展及其应用 |
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| 引用本文： | 侯宇.荧光定量PCR技术研究进展及其应用[J].贵州农业科学,2009,37(6). |
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| 作者姓名： | 侯宇 |
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| 作者单位： | 重庆大学,生物工程学院,重庆,400044 |
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| 基金项目： | 国家科技支撑计划，农业部专项项目 |
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| 摘 要： | 荧光定量PCR技术不仅实现了PCR从定性到定量的飞跃,更以其特异性强、灵敏度高、重复性好、定量准确、速度快、全封闭反应等优点而成为分子生物学研究的重要工具,并用于生物特异核苷酸的检测.主要概述了荧光染料法、TaqMan探针法、分子信标法、Lux Primer 4种荧光定量技术的原理、特点以及荧光定量PCR技术在各方面的应用.
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| 关 键 词： | 荧光探针法 荧光染料法 DNA扩增 定量 |
The Advances and Application of Fluorescence Quantitative Polymerase Chain Reaction(FQ-PCR) |
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| HOU Yu.The Advances and Application of Fluorescence Quantitative Polymerase Chain Reaction(FQ-PCR)[J].Guizhou Agricultural Sciences,2009,37(6). |
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| Authors: | HOU Yu |
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| Institution: | Bioengineering College of Chongqing University;Chongqing 400044;China |
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| Abstract: | Fluorescence Quantitative Polymerase Chain Reaction(FQ-PCR,Real-time PCR or kinetic PCR) became an essential method in molecular biology study and the detection of biological special nucleotide not only because it is quantitative except qualitative but also because its characteristics such as high specificity,high sensitivity,replicable,precise quantification,rapid,and totally closed reaction.This paper reviewed the principles,characteristics,and their application in certain aspects of the 4 fluorescence qu... |
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| Keywords: | fluorescence probe fluorescence dye DNA amplification quantification |
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