| 水稻苗期耐高Cu2+胁迫的QTL定位和上位性分析 |
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| 引用本文： | 沈圣泉,庄杰云,舒庆尧,夏英武.水稻苗期耐高Cu2+胁迫的QTL定位和上位性分析[J].植物营养与肥料学报,2006,12(3):352-357. |
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| 作者姓名： | 沈圣泉 庄杰云 舒庆尧 夏英武 |
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| 作者单位： | 1.浙江大学农业与生物技术学院 浙江杭州310029; |
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| 基金项目： | 国家科技攻关项目;国家科技攻关项目;浙江省科研项目 |
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| 摘 要： | 用珍汕97B/密阳46构建RIL群体及其遗传图谱,对其种子采用沙培法育苗和培养,试验设置2个高浓度(100.mg/L和200.mg/L)Cu2+胁迫处理,以处理20.d后的幼苗相对根长(%)和相对苗高(%)作为苗期耐Cu2+胁迫指标,并用于QTL定位分析。结果表明,试验共检测到苗期耐Cu2+胁迫的主效应QTL.4个,以相对根长为指标,检测到qRCC(r)6(100.mg/L)和qRCC(r)9(200.mg/L),以相对苗高为指标,也检测到qRCC(s)1-2(100.mg/L和200mg/L)和qRCC(r)6-1(200.mg/L),有效基因分散于双亲中。试验还检测到苗期耐Cu2+胁迫的上位性互作8对,以相对根长为指标时,检测到2对互作;以相对苗高为指标时,检测到6对上位性互作。表明水稻苗期耐高浓度Cu2+胁迫,其上位性互作也起到较为重要作用。
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| 关 键 词： | 水稻 RIL群体 QTL定位 上位性效应 耐Cu2+胁迫 |
| 文章编号： | 1008-505X(2006)03-0352-06 |
| 修稿时间： | 2005年3月18日 |
Main and epistasis analysis of QTLs for tolerant to high Cu2+ stress at seedling stage of rice ( Oryza Sativa L. ) |
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| SHEN Sheng-quan,ZHUANG Jie-yun,SHU Qing-yao,XIA Ying-wu.Main and epistasis analysis of QTLs for tolerant to high Cu2+ stress at seedling stage of rice ( Oryza Sativa L. )[J].Plant Nutrition and Fertilizer Science,2006,12(3):352-357. |
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| Authors: | SHEN Sheng-quan ZHUANG Jie-yun SHU Qing-yao XIA Ying-wu |
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| Institution: | 1.College of Agriculture and Biotechnology;Zhejiang University;Hangzhou 310029;China; |
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| Abstract: | The recombinant inbred line(RIL) populations derived from Zhenshan 97B/Miyang 46(ZS97B/MY46) and their genetic linkage maps were employed to map QTLs controlling tolerant to high Cu~(2 ) stress at the seedling stage by culturing the seedling in two concentration of Cu~(2 )(100 mg/L and 200 mg/L) on the sand medium.After 20 days of treatment,the relative root length(%) and relative seedling height(%) was measured as index for tolerant to high Cu~(2 ) stress.The results indicated that a total of four main effect QTLs were detected under high Cu~(2 ) stress.Two were significant for the relative root length,qRCC(r)6(100 mg/L) andqRCC(r)9(200 mg/L),the other two were significant for relative seedling height,qRCC(s)1-2(100 mg/L and 200 mg/L) andqRCC(r)6-1(200mg/L).The positive allele were distributed in both the parents.A total of eight pairs of epistasis were detected for tolerant to high Cu~(2 ) stress.Two were for relative root length;the other six for relative seedling height,indicating that epistasis were important genetic basis for the tolerant to high Cu~(2 ) stress at the seedling stage. |
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| Keywords: | rice RIL population QTLs mapping main effect epistasis effects tolerant to high Cu~(2 ) stress |
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