| 蛋白质免疫印迹快速测定猪肺炎支原体抗原含量 |
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| 引用本文： | 车巧林,胡芳,沈晓红,董彦鹏,靳蒙蒙.蛋白质免疫印迹快速测定猪肺炎支原体抗原含量[J].现代畜牧兽医,2021(1). |
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| 作者姓名： | 车巧林 胡芳 沈晓红 董彦鹏 靳蒙蒙 |
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| 作者单位： | 江苏南农高科技股份有限公司 |
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| 摘 要： | 为建立快速测定猪肺炎支原体抗原含量的方法,试验应用P46单抗建立不同CCU含量的猪肺炎支原体抗原蛋白免疫印迹图谱,测定不同批次的猪肺炎支原体抗原CCU含量,并与Western blot(WB)测定的CCU进行比较。结果显示:曝光5 s,107 CCU/mL含量以上的猪肺炎支原体抗原在46 kDa处有条带,且条带粗细、明暗程度随着CCU含量的增高而增强;中试生产的7批抗原中,有3批抗原CCU和WB 2种方法测定的含量相同,两批抗原测定的含量基本相同;两批抗原测定的含量有差异,但差值在1.5~5.0倍之间。结果表明,WB可用于猪肺炎支原体抗原含量的快速测定。
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| 关 键 词： | 蛋白质免疫印迹 快速测定 猪肺炎支原体 抗原含量 |
Rapid determination of mycoplasma hyopneumoniae antigen content by Western blot |
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| Che Qiaolin,Hu Fang,Shen Xiaohong,Dong Yanpeng,Jin Mengmeng.Rapid determination of mycoplasma hyopneumoniae antigen content by Western blot[J].Modern JOurnal of Animal Husbandry and Veterinary Medicine,2021(1). |
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| Authors: | Che Qiaolin Hu Fang Shen Xiaohong Dong Yanpeng Jin Mengmeng |
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| Institution: | (Jiangsu Nannong Hi-Tech Co.,Ltd.,Jiangsu Jiangyin 214400) |
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| Abstract: | In order to establish a rapid method for the determination of mycoplasma hyopneumoniae(M. hyopneumoniae) antigen content, a P46 monoclonal antibody was used to establish the Western blot(WB) map of M. hyopneumoniae antigen with different content, and then determined the CCU content of M. hyopneumoniae antigen originated from different batches, and compared with the result determined by WB. The results showed that a specific band with a molecular mass of 46 kDa were observed in M. hyopneumoniae(>107 CCU/mL), and the thickness and brightness of the bands increased with the CCU content. Among the seven batches of antigens produced in pilot scale, three batches had the same content determined with the CCU and WB method, and two batches had the essentially similar content. The other two batches had the different content, but the difference was between 1.5~5.0 times. The results indicated that WB could be used for rapid determination of M. hyopneumoniae antigen. |
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| Keywords: | Western blot Rapid determination Mycoplasma hyopneumoniae Antigen content |
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