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| 白肋烟自然调制期间优势菌株的分类鉴定 | |
| 引用本文： | 汪安云,张玉玲,黄琼,祝明亮,柴家荣,林国平.白肋烟自然调制期间优势菌株的分类鉴定[J].甘肃农业大学学报,2009,44(2). |
| 作者姓名： | 汪安云 张玉玲 黄琼 祝明亮 柴家荣 林国平 |
| 作者单位： | 1. 云南省烟草科学研究所,云南,玉溪,653100 2. 云南农业大学,云南,昆明,650201 3. 湖北省烟草科学研究所,湖北,武汉,430000 |
| 基金项目： | 国家烟草专卖局科技计划，云南省烟草专卖局(公司)资助项目 |
| 摘 要： | 以白肋烟‘TN86’为研究对象,在烟株砍收前,对烟叶进行根瘤土壤杆菌属WB5(Agrobaterium tumefa-cien)菌液喷施处理,以喷洒清水为对照,研究了调制期间的优势细菌种群及其变化,测定了优势菌株的硝酸盐还原能力.结果表明:喷洒WB5菌液的烟叶,在调制期的15-50 d的过程中,它们的优势菌株包含有较强的亚硝酸还原能力的假单胞菌和根瘤菌,而对照则没有此类菌株.喷洒WB5菌液,能够改变调制中后期的微生物类群,使具有亚硝酸盐还原能力的菌株种类占据绝对优势;同时分离出1种Paracoccus菌,该菌株具有较强的亚硝酸还原能力. |
| 关 键 词： | 白肋烟 自然调制 优势菌株 16SrDNA TSNA |
Identification of dominating bacteria strain of burley tobacco during air-curing |
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| WANG An-yun,ZHANG Yu-ling,HUANG Qun,ZHU Ming-liang,CHAI Jia-rong,LIN Guo-ping.Identification of dominating bacteria strain of burley tobacco during air-curing[J].Journal of Gansu Agricultural University,2009,44(2). | |
| Authors: | WANG An-yun ZHANG Yu-ling HUANG Qun ZHU Ming-liang CHAI Jia-rong LIN Guo-ping |
| Institution: | WANG An-yun1,ZHANG Yu-ling2,HUANG Qun2,ZHU Ming-liang1,CHAI Jia-rong1,LIN Guo-ping3(1.Yunnan Institute of Tobacco Science,Yuxi 653100,China,2.Yunnan Agricultural University,Kunming 650201,3.Hubei Institute of Tobacco Science,Wuhan 430000,China) |
| Abstract: | Leaves of burley tobacco 'TN86' were sprayed with WB5(Agrobaterium tumefacien) strain isolated from the midrib of thirty samples of burley TRM,while the control was sprayed with water.The leaves were collected every 5 days during air-curing.The dominant bacteria strains were isolated from the collected leaves by general isolation method and were identified by morphological,physio-biochemical characteristics,and 16S rDNA sequence analysis method.The main difference in bacteria strains was that those isolated... |
| Keywords: | 16SrDNA TSNA |
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